MOUNIRA Ariech

Allal omayma riham , Azzi aya, Faid ouiam

Production d'édulcorant à Base de plantes

Sammah Affef , Zeroual Cherifa

Formulation d'une pommade antiinflammatoire et antiseptique

Ferrat Tassadit , Milat Medjda, Ghouini Merouan

Formulation d'une crème antifongique naturelle à base de plantes médicinales locales

Khanache Siham , Zitouni Ghalya

Formulation d'un remède des toxi-infections alimentaires

khlif Khawla

Valorisation des débris végétaux en bioplastiques

Abbassi Anissa

Développement d'outils de contrôle de la qualité microbiologique

Rajai Hadjira

Formulation d'un produit antifongique contre les champignons phytopathogènes

Akrib Omayma

An Intelligent Colorimetric Patch for Monitoring the Freshness of Protein-Rich Foods

Djoudi Anfal

Valorisation de bio-source dans la prévention bucco-dentaire

BEN BAKA Kahia

Développement d'un système de caractérisation automatique des bactéries pathogènes

bouhacida chawki , touatit belkacem, aribi alae

les plantes médicinales en pastille contre la bronchite, la toux et les angines d'origine bactériennes

ben hlima nadjet , ben ria nawel, bakir radia

formulation des préparations dermatologiques a base de probiotiques

Maouche Dalel , Gherbi Nora

evaluation des infections urinaire dansla région de M'sila

Ahmed Fatma Zahrae , Ben Hamida Hadjer

Détection des résidus d'antibiotiques dans la viande du poulet de chair dansla région du M'sila

Kahlane Meriem , Khalfet Manel

Effet de la salinité sur la stabilité des biosurfactants produits par Halomonas caseinilytica sp. en présence de gasoil.

Benzergua Saida , Fordjen Nadia

Utilisation des biosurfactants produits par Halomonas casernilytica sp pour le traitement d'un sol sableux contaminé par les hydrocarbures

Zerouak Nour El Houda

Détermination des propriétés des biosurfactants produits par Halomonas caseinilytica, isolées des sols salins du Chott El Hodna-M'sila.

Bentoumi Meriem , Deneche Imène

Screening des souches d'Haloarcheae capables de produire les biosurfactant

Ramdani Zahia

Caractérisation phénotypique des Archaea halophiles isolées du Chott El Hodna -M'sila-

Mohamed nour Abdelkerim , Mokhtari Bilal, Cherrid Nacerddine and Seghiour Rabeh

les Archaea halophiles et leurs milieux extremes

Sahnoune Dalel , Chalbabe Fatima Zahrae

l'hépatite virale, médicamenteuse et auto-immune

Biosurfactants are surfactant molecules produced by certain microorganisms (bacteria, yeasts, and fungi). Their nature as well as their surfactant power are strongly dependent on the type of microorganism, the species as well as the nutrient substrate available for cell development. Among the different biosurfactants identified today, we find glycolipids, lipopeptides, phospholipids, neutral lipids, fatty acids or lipopolysaccharides. Just like their chemical synthesis counterparts, they can have emulsifying, foaming, wetting or even dispersing properties, but also more specific properties. Currently, biosurfactants are of great interest in various areas of life thanks to their biodegradability and low toxicity, the objective of this study revolves around the production and characterization of biosurfactants from bacteria isolated from different soil samples, all isolates purified by successive subcultures of well-separated and macroscopically distinct colonies were checked for catalase, oxidase reaction and examined microscopically after gram staining. 24 isolates were screened for biosurfactant production on nutrient agar using the following tests: drop collapse test, oil dispersion test, emulsion stability test (ES%). The drop collapse test is based on the destabilization of an oil droplet by surfactants in a 96-well microplate. The oil dispersion test is based on the dispersing activity of bacteria in a layer of the oil phase. Whereas, the calculation of the emulsification index E24 makes it possible to verify the ability to form an emulsion between a hydrophobic phase and a hydrophilic phase. Only three strain was found to be efficient. key words: biosurfactants, surface tension, screening, isolation, bacteria.

Citation

Zoulikha KERMICHE , Mounira ARIECH , ,(2024-11-02), isolation and screening of bacteria producing biosurfactants,The First National Seminar on the VALORISZATION OF MICROBIAL OROGIN PRODUCTS VMOP’24- Université des Frères Mentouri Constantine 1,Université des Frères Mentouri Constantine 1

Biosurfactants are surfactant molecules produced by certain microorganisms (bacteria, yeasts, and fungi). Their nature as well as their surfactant power are strongly dependent on the type of microorganism, the species as well as the nutrient substrate available for cell development (Chen et al., 2007). Among the different biosurfactants identified today, we find glycolipids, lipopeptides, phospholipids, neutral lipids, fatty acids or lipopolysaccharides. Just like their chemical synthesis counterparts, they can have emulsifying, foaming, wetting or even dispersing properties, but also more specific properties (antibiotic properties). Some of these functional properties can, moreover, be preserved under extreme conditions of use such as acidic pH, high temperature, and salinity (Meylheuc et al., 2001). Given their potential and their safety, they are today used in different fields of application such as the environment, the oil industry, agronomy or even cosmetology and should quickly find their place in new sectors. applications such as the food, pharmaceutical and medical industries. The objective of this study revolves around the production and characterization of biosurfactants from bacteria isolated from different samples

Citation

Zoulikha KERMICHE , Mounira ARIECH , Abed Arabi: arabi.abed@univ-mosta.dz, ,(2024-06-26), contribution of valorization of biosurfactants of bacterial origin,1 st International Seminar of AgriFood and Industrial Biotechnology ISABI 2024- Université Djilali Bounaama Khemis Miliana,Université Djilali Bounaama Khemis Miliana

ABSTRACT: Galactomannans are the main component of locust bean gum from the fruit of the carob tree, Ceratonia siliqua L. They are a reserve of polysaccharides, found in the translucent endosperm of the seeds. They are designated as the best gels with thickening capacity and are, therefore, widely used as a natural food additive (E410) in many food, pharmaceutical and cosmetic preparations. In this study, we aim to exploit this gelling property of carob galactomannans in the microencapsulation of lactic bacteria in order to protect them from the negative effects of simulated digestive conditions. Two beneficial bacteria are used: Lactobacillus rhamnosus LBRE-LSAS and Bifidobacterium animalis subsp. lactis Bb12. Their survival in the free state or encapsulated in pure carob galactomannan gel combined with xanthan, was determined after residence in simulated in vitro digestive conditions (gastric: pH 2, pepsin 3 g/l and intestinal: bile 0.3%: W/V, pH 6.5. The results obtained show that gel encapsulation of carob galactomannans combined with xanthan improves the survival of these two beneficial strains to simulated digestive hostilities. the loss under gastric conditions 36.79% (3.55 log CFU/mL) for the non-encapsulated cells and only 12% (1.2 log CFU/mL) for the encapsulated ones. However, galactomannans alone do not appear to be effective in keeping a minimum of 106 bacterial cells viable when confronted with the hostile conditions of the digestive tract where they will be called upon to exert their positive effect on health. Keywords: Galactomannans; Xanthan; Encapsulation; Survival; Digestive Hostilities. 1. INTRODUCTION Probiotics are live microorganisms which, when administered in sufficient quantities, confer beneficial health effects on the host [1,2]. To exert health effects, it is recommended to ingest foods containing at least 106-107 colony forming units (CFU)/g of viable probiotics [3]. One of the major difficulties in the use of probiotics is their low viability during the crossing and stay in the different compartments of the digestive system where a large part of the cells is lost or inhibited by the bile secretions. Indeed, to be effective in their role, these micro-organisms must then colonize the intestine and multiply there. The low tolerance to acidity of certain bacterial species requires the implementation of means of protection and preservation of their integrity and survival in highly acidic environments such as the gastric

Citation

Mounira ARIECH , , (2022-09-02), Encapsulation effects of galactomannans combined with xanthan on the survival of two lactic strains under simulated digestive hostilities, European Journal of Biological Research, Vol:0, Issue:3, pages:9, Zenodo

Abstract Food neophobia describes the reluctance to taste an unfamiliar food, or a familiar food but presented in a new form. It appears to be a normal phase of development, frequently encountered in children. In Algeria, this type of study is rare, which justifies our commitment to study this subject. Our survey was carried out on a sample of 746 schoolchildren, aged 6 to 10 years old, from the M'sila region, including 360 girls and 386 boys to estimate the rate and level of food neophobia among these children and to define the influence of their surroundings on this food behavior by answering two basic questionnaires. The results of the statistical analyses show that 12.06 % of the children in the population were neophobic and no significant difference appeared in the level of food neophobia according to gender, however, food neophobia evolves progressively with age. Multiple correspondence factor analysis (MCFA) indicates that the media influences the choice of neophobic children. Key words: Food neophobia, food, schoolchildren

Citation

Mounira ARIECH , , (2021-05-31), INFLUENCE OF ENVIRONMENTAL FACTORS ON FOOD NEOPHOBIA IN CHILDREN AGED 6 TO 10 YEARS OLD, Natural Resources and Sustainable Development, Vol:11, Issue:1, pages:16, Editura Universităţii din Oradea , Romania

Background: Bioremediation processes are negatively affected by the low aqueous solubility of some contaminants; therefore their bioavailability may be enhanced by the addition of surfactants. These compounds are organic molecules that can be chemically and biologically produced. Surfactants contain both hydrophilic and hydrophobic groups, therefore reducing surface and interfacial tensions of immiscible fluids and increasing the solubility and sorption of hydrophobic organic and inorganic compounds. The aim of our work is the decontamination of a sandy soil artificially contaminated by hydrocarbons using the biosurfactants produced by the strain Natrinema gari sp. Materials/Methods : The effectiveness of cell‐free biosurfactant, derived from the culture medium at the end of fermentation was investigated for the removal of oil from contaminated sandy soils, with differents concentrations (1/2 CMC, CMC and 2 CMC) and at different time of exposure (10 min, 120 min and 1440 min). The evaluation of biosurfactant action in the removal of oil from different contaminated sandy soil was performed using a statistical experimental. Results: The strain Natrinema gari sp. is a potent producer of biosurfactant wich is able to eliminate oil with a percentage of 88 % for a contact time of 10 min, 85% for 120 min and 89 % for 1440 min, but the cell culture was the best in remobilization than biosurfactant crude. For a contact time of 10 min, the strain has an oil removal percentage of 98%, 96% for 120 min and 92% for 1440 min. The chemical surfactant (SDS) does not remove crude oil but it changes their property. The biosurfactant produced by this strain, remain able of decontaminating sands polluted by oil, using the bacterial cultures with a percentage of 100 % or 2CMC of biosurfactant with a percentage of 98%. Conclusion: The effectiveness of biosurfactants produced by Natrinema gari CH5, suggests that it may be a preferable remediation method for the oil contamination soils. Keywords : Natrinema gari sp., biosurfactant, crude oil, decontamination, sandy soil.

Citation

Mounira ARIECH , ,(2019), treatment of sandy soil contaminated with hydrocarbons using biosurfactants produced by a halophilc Archaea,The 3rd international congress of biochimistry and microbiology applied technologies. BMAT 2019,Hammamet-Tunisia

Background: Bioremediation processes are negatively affected by the low aqueous solubility of some contaminants; therefore their bioavailability may be enhanced by the addition of surfactants. These compounds are organic molecules that can be chemically and biologically produced. Surfactants contain both hydrophilic and hydrophobic groups, therefore reducing surface and interfacial tensions of immiscible fluids and increasing the solubility and sorption of hydrophobic organic and inorganic compounds. The aim of our work is the decontamination of a sandy soil artificially contaminated by hydrocarbons using the biosurfactants produced by the strain Natrinema gari sp. Materials/Methods : The effectiveness of cell‐free biosurfactant, derived from the culture medium at the end of fermentation was investigated for the removal of oil from contaminated sandy soils, with differents concentrations (1/2 CMC, CMC and 2 CMC) and at different time of exposure (10 min, 120 min and 1440 min). The evaluation of biosurfactant action in the removal of oil from different contaminated sandy soil was performed using a statistical experimental. Results: The strain Natrinema gari sp. is a potent producer of biosurfactant wich is able to eliminate oil with a percentage of 88 % for a contact time of 10 min, 85% for 120 min and 89 % for 1440 min, but the cell culture was the best in remobilization than biosurfactant crude. For a contact time of 10 min, the strain has an oil removal percentage of 98%, 96% for 120 min and 92% for 1440 min. The chemical surfactant (SDS) does not remove crude oil but it changes their property. The biosurfactant produced by this strain, remain able of decontaminating sands polluted by oil, using the bacterial cultures with a percentage of 100 % or 2CMC of biosurfactant with a percentage of 98%. Conclusion: The effectiveness of biosurfactants produced by Natrinema gari CH5, suggests that it may be a preferable remediation method for the oil contamination soils. Keywords : Natrinema gari sp., biosurfactant, crude oil, decontamination, sandy soil.

Citation

Mounira ARIECH , ,(2019), Bioremediation of soil contaminated with oil using biosurfactants produced by a halophilic Archaea,1er séminaire national: l'apport des biotechnologies sur la protection de l'environnement.,M'sila-ALGERIA

Abstract Biosurfactant (BS) are a type of surface-active molecules that reduce the interfacial tension between two liquids, or that between a liquid and a solid. Their unique property like, nontoxic, easily biodegradable, eco-friendly and high stability, and wide variety of industrial application makes them highly useful group of chemical compound. BS are produced from variety of microorganisms. The objective of this study was to isolate and characterize the BS producingby Natrinema gari sp., halophilic archeon isolated from salin soils of Chott El Hodna, M'sila- Algeriaand identified by 163 rRNA sequencing. BS production ability was evaluated by carrying out drop collapse and oil spreading tests, measurement of surface tension and estimation of emulsification activity. Properties of this BS were determined like charactrization of BS by TLC and GC-MS methods. It was analysed and identified as lipid-biosurfactant and GC-MS analysis revealed that they are predominantly made up of a benzene with a percent age of 50Yo, and a mixture of fatly acids (palmitic acid, stearic acid and linoleic acid). Keywords Natrinema gari sp., biosurfactants, Haloarchaea, GC-MS, faffy acids, screening.

Citation

MouniraARIECH , ,(2018); lsolation and Characterization of biosurfactant produced by Natrinema gari sp.,a halophilic archeon isolatedfrom salin soils of Chott El Hodna-M,sila, Algeria in :Exploring Miuoorganisms: Recent Advances in Applied Microbiology_,,BrownWalker Press

A halophilic, Gram-negative bacterial strain, designated CH7, which was isolated from saline soils of Chott El Hodna, M’sila-Algeria, was subjected to a polyphasic taxonomic study. Overall, the phenotypic, genotypic and phylogenetic results demonstrate that strain CH7 represents a member of the genus Halomonas. The 16S rRNA gene sequence of this strain shows highest similarity of 99.8 % with Halomonas caseinilytica DSM 23509T . This potent biosurfactant producer and diesel degrader, was obtained after screening involving ; drop collapsing method (DCM), oil-spreading method (OSM), surface tension mesurement (STM) and emulsification index (E24). This strain was found to be a potent producer of biosurfactant in SG broth containing diesel oil as the carbon source and found to be able to degrad diesel oil. The quantitative biodegradation efficiency (BE%) of diesel was 45.88%, over a period of 15 days, calculated by gas chromatography mass spectrum analysis (GC-MS). The biosurfactant showed high physicochemical properties in terms of the surface activities, and reducing the surface tension to 23.00 ± 0.5 mN.m-1 and its critical micelle concentration (CMC) was found to be 46 mg.L-1, with emulsification index of 82 ± 0.5 %. The biosurfactant showed thermal and pH stability regarding stability emulsion, also under salinity ranges. Key words Biosurfactants, biodegradation, diesel, emulsion, Halomonas, halophilic bacteria, surface tension.

Citation

Mounira ARIECH , ,(2018), Detremination of properties and applications of biosurfactant produced by Halomonas caseinylitica sp., a halophilic bacterium isolated from saline soils of chott el hodna, algeria.,The 2nd international congress of biochimistry & microbiology applied technologies BMAT 2018,Hammamet-Tunisia

Résumé Les Haloarchaea sont des Archaea halophiles, inclus dans un ordre et une famille uniques, nommés Halobacteriales et Halobacteriaceae, respectivement. Elles survivent dans les milieux à très fortes concentrations en sel (NaCl>1,5M). Actuellement, les Haloarchaea présentent un grand intérêt grâce à leur capacité de produire les molécules bioactives telles que les biosurfactants qui ont des applications très importantes dans divers domaines de la vie ; vue les propriétés fonctionnelles de ces molécules, dont les propriétés d’emulsification et la réduction de la tension superficielle. Dans le présent travail, nous avons effectué un screening de la capacité de produire les biosurfactants par six isolats des bactéries halophiles extrêmes, isolées des sols salins de Chott El Hodna-M’sila, en utilisant quatre tests. Tous les résultats montrent que les isolats CH2 et CH5, sont actifs et capables de produire à la fin de la phase stationnaire de croissance, des biosurfactants extracellulaires, qui demeurent stables aux variations de la température,du pH et de la salinité . Les concentrations micellaires critiques déterminées sont : CMCCH2=280 mg/l et CMCCH5=80 mg/l. L’optimisation des conditions physico-chimiques et nutritionnelles qui influencent la production des biosurfactants a donné les meilleurs résultats à savoir un % RTS=68.54±0.7% pour l’isolat CH2 ; une valeur qui correspond à une TSCH2=20.66±0.1 mN.m-1. De même pour l’isolat CH5, les meilleurs résultats obtenus sont les suivants : %RTSCH5=48.52±0.5% ; une valeur qui correspond à une TSCH5=35.67±0.2 mN.m-1. L’identification de ces molécules par GC-MS a permis de conclure que les biosurfactants produits par l’isolat CH2 et CH5 sont constitués essentiellement d’acides gras. Ces biosurfactants demeurent capables de décontaminer les sables pollués par le pétrole, en utilisant le moût de fermentation ou les biosurfactants à 2 CMC avec un pourcentage d’élimination de 100% avec un pourcentage d’ellimination de 98% Mots Clés : Archaea halophiles biosurfactants screening tension superficielle

Citation

MouniraARIECH , ,(2018); Identification des Archaea halophiles isolés du Chott El Hodna M’sila, productrices des biomolécules,Université Ferhat Abbas Sétif1,

Dans le cadre de la valorisation des produits issus de la recherche scientifique, la Direction Générale de la Recherche Scientifique et du Développement Technologique (DGRSDT) aorganisé la deuxième édition du Salon National des Produits de la Recherche les 2, 3 et 4 juillet 2018 au Palais des Expositions, Pins Maritimes – Alger.

Citation

Mounira ARIECH , ,(2018), biosurfactant polystable utile dans la bioremédiation des milieux pollués par le pétrole ».,salon national des produits de la recherche,Alger-Algerie

قي اطار فعاليات تظاهرة الجامعة الخريقية تحت شعار :"نحو شراكة بين الجامعة و المؤسسات الاقتصادية" و التي نظمتها جامعة المسيلة بالتعاون مع غرفة التجارة و الصناغة-المسيلة و منتدى رؤساء المؤسسات-المسيلة

Citation

Mounira ARIECH , ,(2018), "Les détergents écologiques".,l'université d'Automne.-Université de M'sila,Université de M'sila

Abstract Biosurfactant (BS) are a type of surface-active molecules that reduce the interfacial tension between two liquids, or that between a liquid and a solid. Their unique property like, nontoxic, easily biodegradable, eco-friendly and high stability, and wide variety of industrial application makes them highly useful group of chemical compound. BS are produced from variety of microorganisms. The objective of this study was to isolate and characterize the BS producingby Natrinema gari sp., halophilic archeon isolated from salin soils of Chott El Hodna, M'sila- Algeriaand identified by 163 rRNA sequencing. BS production ability was evaluated by carrying out drop collapse and oil spreading tests, measurement of surface tension and estimation of emulsification activity. Properties of this BS were determined like charactrization of BS by TLC and GC-MS methods. It was analysed and identified as lipid-biosurfactant and GC-MS analysis revealed that they are predominantly made up of a benzene with a percent age of 50Yo, and a mixture of fatly acids (palmitic acid, stearic acid and linoleic acid). Keywords Natrinema gari sp., biosurfactants, Haloarchaea, GC-MS, faffy acids, screening.

Citation

Mounira ARIECH , ,(2017), lsolation and Characterization of biosurfactant produced by Natrinema gari sp.,a halophilic archeon isolatedfrom salin soils of Chott El Hodna-M,sila, Algerii,BIOMICROWORLD 2017 CONFERENCE,Madrid-Spain

L’expérimentation animale consiste à utiliser des animaux comme substitut ou « modèle », pour mieux comprendre la physiologie d'un organisme et ses réponses à divers facteurs (alimentation, environnement, agents pathogènes) ou substances (pour en tester, vérifier ou évaluer l'efficacité, l'innocuité ou la toxicité), et tout particulièrement pour tenter de prévoir ce qui se passe chez l'Homme.

Citation

Mounira ARIECH , ,(2017), Les bonne pratiques d'utilisation des animaux de laboratoire,Les bonne pratiques d'utilisation des animaux de laboratoire".,Université de m'sila

Biosurfactants are surface active agents with broad range commercial applications in various industriesand have considerable advantages over their chemical counterparts. In this study, drop collapse assay,oil spreading assay, emulsification assay and surface tension measurement were compared for theirreliability and ease of use to screen biosurfactant producing six extremely halophilic bacteria isolatedfrom saline soil of Chott El Hodna-M’sila (Algeria), which is considered as a thalassohaline environment.Results from screening methods revealed that, CH2 and CH5 strains are potential candidates forbiosurfactant production. Among these, there was a very strong negative linear correlation between theemulsification index and surface tension (rs= - 0.987) and a strong negative linear correlation betweenthe oil spreading technique and surface tension (rs= - 0.971) and a weaker negative correlation betweenthe drop collapse method and surface tension (rs= - 0.807), suggesting that strains highly active in anyone of these methods were active in other three methods. As every method has its advantages anddisadvantages, a combination of different methods is appropriate for a successful screening.Key words: Biosurfactant, drop collapse, emulsification stability, extremely halophilic bacteria, oil spreading,surface tension.

Citation

Mounira ARIECH , , (2015-05-25), Assessment of four different methods for selecting biosurfactant producing extremely halophilic bacteria, AFRICAN JOURNAL OF BIOTECHNOLOGY, Vol:14, Issue:21, pages:10, Nairobi: Academic Journals.

Biosurfactants are surface active agents with broad range commercial applications in various industries and have considerable advantages over their chemical counterparts. In this study, drop collapse assay, oil spreading assay, emulsification assay and surface tension measurement were compared for their reliability and ease of use to screen biosurfactant producing six extremely halophilic bacteria isolated from saline soil of Chott El Hodna-M’sila (Algeria), which is considered as a thalassohaline environment. Results from screening methods revealed that, CH2 and CH5 strains are potential candidates for biosurfactant production. Among these, there was a very strong negative linear correlation between the emulsification index and surface tension (rs= - 0.987) and a strong negative linear correlation between the oil spreading technique and surface tension (rs= - 0.971) and a weaker negative correlation between the drop collapse method and surface tension (rs= - 0.807), suggesting that strains highly active in any one of these methods were active in other three methods. As every method has its advantages and disadvantages, a combination of different methods is appropriate for a successful screening. Key words: Biosurfactant, drop collapse, emulsification stability, extremely halophilic bacteria, oil spreading, surface tension.

Citation

Mounira ARIECH , , (2015), Assessment of four different methods for selecting biosurfactant producing extremely halophilic bacteria, African Journal of Biotechnology, Vol:14, Issue:21, pages:1764-1772, Academic journals

Biosurfactants are surface active agents with broad range commercial applications in various industries and have considerable advantages over their chemical counterparts. In this study, drop collapse assay, oil spreading assay, emulsification assay and surface tension measurement were compared for their reliability and ease of use to screen biosurfactant producing six extremely halophilic bacteria isolated from saline soil of Chott El Hodna-M’sila (Algeria), which is considered as a thalassohaline environment. Results from screening methods revealed that, CH2 and CH5 strains are potential candidates for biosurfactant production. Among these, there was a very strong negative linear correlation between the emulsification index and surface tension (rs= - 0.987) and a strong negative linear correlation between the oil spreading technique and surface tension (rs= - 0.971) and a weaker negative correlation between the drop collapse method and surface tension (rs= - 0.807), suggesting that strains highly active in any one of these methods were active in other three methods. As every method has its advantages and disadvantages, a combination of different methods is appropriate for a successful screening. Key words Biosurfactant, halophilic bacteria, drop collapse assay, oil spreading assay, emulsification assay, surface tension.

Citation

Mounira ARIECH , ,(2015), Isolation of extremely halophilic bacteria able to produce biosurfactant.,The 1st international conference micribiol ecology : towards new challenges.,Sétif-ALGERIA

Biological control such as the use of plant extracts has emerged as promising option to the phenomena of microorganisms resistance to chemical. Several constituent of essential oil have been studied for their biological activity including antibacterial and antifungal activity. In this study the effect of Saccocalyx satureioides essential oil with different concentrations was test against the growth of some bacteria and moulds. Saccocalyx satureioides was treated by hydrodistillation in order to obtain its essential oils. The method gave an output of 1.8%. The test of the inhibiting capacity of these EO, diluted to 50% and 33.33%, on the growth of some bacteria and moulds presented a strong antimicrobial activity reaching more than 50%.The essential oil of Saccocalyx satureioides a broad spectrum of activity as it inhibits both the growth of bacteria that moulds and yeasts. Its antimicrobial activity is mainly a function of its chemical composition. It acts by preventing the growth of bacteria and yeast, whereas it inhibits spore germination, elongation of mycelium, sporulation and production of exudates from the moulds. Key words Antimicrobial activity, Essential oils, Hydrodistillation, Saccocalyx satureioides.

Citation

Mounira ARIECH , ,(2014), Antimicrobial activity of Saccacolyx satureioides essentiel oil and extract obtained by hydrodistillation.,1er séminaire national:”Etat des lieux, conservations et possibilités de valorisation des ressources biologiques dans l’Est algérien,Mila-Algeria

Abstract : Biosurfactants are the surface-active molecules synthesized by microorganisms. The present study was focused on screening of biosurfactant producing halophilic bacteria. Total three methods were employed to test the biosurfactant production in six halophilic strains by drop collapsing method and oil spreading method. The results of the latest method were ranged from 78.73 ± 02.85 - 246.22 ± 08.79 mm2. And finally, the emulsifying capacity of these strains was evaluated by the E24 emulsification index and it ranged from 08.68 -80.00 %. With the advantage of environmental compatibility. In conclusion, interest in biosurfactants has led to the developement of a multitude of methods for the screening of biosurfactant producing strains. As every method has its advantages and disadvantages, a combination of different methods is appropriate for a successful screening. Keywords: Biosurfactant; Drop collapsing test; Emulsification index ; Oil displacement test.

Citation

Mounira ARIECH , ,(2014), Assessement of thre screening methods for selecting biosurfactant producing halophilic bacteria.,Les 20ème journées nationals de microbiologie.,Jijel-ALGERIA

Drop collapse assay, oil spreading assay, emulsification assay and surface tension measurement were compared for their reliability and ease of use to screen biosurfactant producing six extremly halophilic archaea isolated from saline soil of Chott El Hodna-M’sila (Algeria), which is considered as a thalassohaline environment. Results from screening methods revealed that, CH2 and CH5 strains are potential candidates for biosurfactant production. Among these, there was a very strong negative linear correlation between the emulsification index and surface tension (rs= - 0.987) and a strong negative linear correlation between the oil spreading technique and surface tension (rs= - 0.971) and a weaker negative correlation between drop collapse method and surface tension (rs= - 0.807), suggesting that strains highly active in any one of these method were active in other three methods. As every method has its advantages and disadvantages, a combination of different methods is appropriate for a successful screening. Keywords Biosurfactant. Drop collapse. Emulsification stability. Halophilic Archaea. Oil spreading. Surface tension.

Citation

Mounira ARIECH , ,(2014), Evaluation of screening methods for biosurfactant producing halophilic bacteria.,Le 1er colloque international de biotechnologie végétale et microbienne.,Oran-ALGERIA

La diversité et la densité des bactéries halophiles dans les sols salins et les eaux salées de Chott El Hodna (M'sila), ont été étudiés, en incluant trois sites de prélèvement pour les eaux et quatre sites de prélèvement pour le sol, dans des systèmes écologiques différents. Les propriétés physico - chimiques du sol et des eaux ont été analysées. Les échantillons collectés à partir des saumures salines de Chott El Hodna, ont été trouvés thalassohalines, en raison de la dominance des ions de sodium et des chlorures avec un pH de 8,0. Les densités des bactéries halophiles faibles, modérées et extrêmes, ont été mesurées en utilisant un milieu de culture salé contenant 3%, 8%, et 25% respectivement. La densité des bactéries halophiles faible était la plus élevée, suivie par les bactéries halophiles modérées puis les bactéries hyper halophiles. Vingt isolats d’archéobactéries hyper halophiles et une bactérie halotolérante ont été sélectionnés pour la caractérisation, en adoptant la comparaison de leurs caractéristiques phénotypiques avec d’autres espèces déjà identifiés dans le Manuel de Bergey pour la systématique en bactériologie. Ces isolats peuvent être identifiés comme appartenant à sept genres et dix espèces. Les genres sont: Halopherax, Haladaptatus, Halococcus, Natrinema, Haloplanus, Natrialba. Halomonas. La production du beta-carotène par les espèces chromogènes (Halopherax volcanii. Halococcus morrhuae. Halococcus qingdaonensis. Natrinema pallidum. Natrinema pellirubrum. Haloferax mediterrane.), a été révélée par la méthode de chromatographie sur couche mince (CCM). La caractérisation de ces isolats, en utilisant une combinaison des caractéristiques phénotypiques, une analyse des acides gras de la paroi, et l’analyse des séquences d’ARNr16S, est recommandée pour une utilisation future de ces isolats pour des applications en biotechnologie. Mots clés : Chott El Hodna, Archéobactéries hyper halophiles, Caroténoïdes, Eaux salées, Sols salins, Thalassohaline.

Citation

Mounira ARIECH , ,(2013), Etude de la diversité des bactéries halophiles du chott el hodna, M’sila.,Le 1er colloque national sur les zones humides (CNZH1).,M'sila-ALGERIA

Abstract : The current study describes the extracellular hydrolytic activities of halophilic archaea isolated from salin soils of Chott El Hodna (M’sila). The estimated number of grown colonies was approximately 38 per gram of saline soil on the surface of the medium containing 25% NaCl. The investigated strains, randomly selected from the colonies observed on the plate, showed at least one of the tested extracellular hydrolytic activities and one strain hydrolized six out of seven tested substrates. Our investigations showed that hydrolytic activities for Tween 80, casein and gelatin are predominant among the isolated strains although the NaCl concentration varies up to 25%. The presence of combined hydrolytic activities in some isolated strains could be an advantage to use in some biotechnological applications in various fields of industry or agriculture. To the author’s knowledge, this is the first study on the extracellular hydrolytic properties of halophilic archaea isolated from Chott El Hodna, which was classified as an important international wetland by the RAMSAR Convention. Key words: Extremozymes, halophilic archaea,hydrolytic activities, halophilic enzymes, salt.

Citation

Mounira ARIECH , ,(2013), Hydrolytic enzymes of halophilic archaea isolated from chott el hodna m’sila (algeria) and their economic values.,4th International Workshop on Industrial Biotechnology,Telemcen-ALGERIA

Abstract : The current study describes the extracellular hydrolytic activities of halophilic archaea isolated from salin soils of Chott El Hodna (M’sila). The estimated number of grown colonies was approximately 38 per gram of saline soil on the surface of the medium containing 25% NaCl. The investigated strains, randomly selected from the colonies observed on the plate, showed at least one of the tested extracellular hydrolytic activities and one strain hydrolized six out of seven tested substrates. Our investigations showed that hydrolytic activities for Tween 80, casein and gelatin are predominant among the isolated strains although the NaCl concentration varies up to 25%. The presence of combined hydrolytic activities in some isolated strains could be an advantage to use in some biotechnological applications in various fields of industry or agriculture. To the author’s knowledge, this is the first study on the extracellular hydrolytic properties of halophilic archaea isolated from Chott El Hodna, which was classified as an important international wetland by the RAMSAR Convention. Key words: Extremozymes, halophilic archaea,hydrolytic activities, halophilic enzymes, salt.

Citation

Mounira ARIECH , ,(2013), Proteolytic activity from some strains of Haloarchaea.,Journée des masters et doctoriales.,Sétif-ALGERIA

Biological control such as the use of plant extracts has emerged as promising option to the phenomena of microorganisms resistance to chemical. Several constituent of essential oil have been studied for their biological activity including antibacterial and antifungal activity. In this study the effect of Saccocalyx satureioides essential oil with different concentrations was test against the growth of some bacteria and moulds. Saccocalyx satureioides was treated by hydrodistillation in order to obtain its essential oils. The method gave an output of 1.8%. The test of the inhibiting capacity of these EO, diluted to 50% and 33.33%, on the growth of some bacteria and moulds presented a strong antimicrobial activity reaching more than 50%.The essential oil of Saccocalyx satureioides a broad spectrum of activity as it inhibits both the growth of bacteria that moulds and yeasts. Its antimicrobial activity is mainly a function of its chemical composition. It acts by preventing the growth of bacteria and yeast, whereas it inhibits spore germination, elongation of mycelium, sporulation and production of exudates from the moulds. Key words Antimicrobial activity, Essential oils, Hydrodistillation, Saccocalyx satureioide

Citation

Mounira ARIECH , ,(2013), Antimicrobial activity of Saccocalyx satureioides essential oil and extract obtained by hydrodistillation.,The 1st International plants, Health and Environment,M'sila-ALGERIA

La diversité et la densité des bactéries halophiles dans les sols salins et les eaux salées de Chott El Hodna (M'sila), ont été étudiés, en incluant trois sites de prélèvement pour les eaux et quatre sites de prélèvement pour le sol, dans des systèmes écologiques différents. Les propriétés physico - chimiques du sol et des eaux ont été analysées. Les échantillons collectés à partir des saumures salines de Chott El Hodna, ont été trouvés thalassohalines, en raison de la dominance des ions de sodium et des chlorures avec un pH de 8,0. Les densités des bactéries halophiles faibles, modérées et extrêmes, ont été mesurées en utilisant un milieu de culture salé contenant 3%, 8%, et 25% respectivement. La densité des bactéries halophiles faible était la plus élevée, suivie par les bactéries halophiles modérées puis les bactéries hyper halophiles. Vingt isolats d’archéobactéries hyper halophiles et une bactérie halotolérante ont été sélectionnés pour la caractérisation, en adoptant la comparaison de leurs caractéristiques phénotypiques avec d’autres espèces déjà identifiés dans le Manuel de Bergey pour la systématique en bactériologie. Ces isolats peuvent être identifiés comme appartenant à sept genres et dix espèces. Les genres sont: Halopherax, Haladaptatus, Halococcus, Natrinema, Haloplanus, Natrialba. Halomonas. La production du beta-carotène par les espèces chromogènes (Halopherax volcanii. Halococcus morrhuae. Halococcus qingdaonensis. Natrinema pallidum. Natrinema pellirubrum. Haloferax mediterrane.), a été révélée par la méthode de chromatographie sur couche mince (CCM). La caractérisation de ces isolats, en utilisant une combinaison des caractéristiques phénotypiques, une analyse des acides gras de la paroi, et l’analyse des séquences d’ARNr16S, est recommandée pour une utilisation future de ces isolats pour des applications en biotechnologie. Mots clés : Chott El Hodna, Archéobactéries hyper halophiles, Caroténoïdes, Eaux salées, Sols salins, Thalassohaline.

Citation

Mounira ARIECH , ,(2011), Identification des archéobactéries hyper halophiles du chott el hodna, M’sila,Les 17èmes journées nationales de microbiologie.,Sétif-ALGERIA