ABDERRAHIM Benslama

CHEBABHA Chahira , CHABI Ibtissam, MAKHLOUF Farida

Etude de l’activité antibactérienne des champignons endophytes isolé de Retama retam

Benzemmouri Kheira , Guemra Lina Loudjeyne, Khezzari Khaoula

Étude de l’activité antioxydante et antibactérienne de propolis

BEN NACEIR Chahinez , BAATOUCHE Chahinez, BOUABDALLAH Dounya

Essai d’élaboration d’un yaourt fonctionnel sucré par la poudre des graines de caroube

Gasri Fatiha , Hamadi Safa

Evaluation de l’activité antioxydante de l’extrait méthanolique de la plante Genista quadriflora

DELLALI SAADIA , TORCHI IMANE

Etude ethnopharmacologique et phytochimique de pallenis spinosa

Zoubida Charef , Amina Himeur

Evaluation de l’activité antioxydante des extraits d’Ammi visnaga

Kharfi Nour Elimane , Rezgui Khouloud

L’effet de pasteurisation sur les caractéristiques physicochimiques et microbiologiques de lait de vache

Lekhel Nadjette

Polyphénols totaux et activité antibactérienne des extraits de la plante médicinale Ammi visnaga L.

Zouid Imen , Mokarne El-Roumayssa

Évaluation de l'activité antibactérienne des extraits de quatre plantes médicinales

CHIRANE Aymene , MERZOUD Youssouf

Evaluation de l’activité antibactérienne des champignons endophytes isolés à partir Artemisia herba alba

Senecio perralderianus belongs to the family of Asteraceae and is only found in Algeria. Some species of this family are used to heal gastrointestinal issues in conventional medicine for their antioxidant and anti-inflammatory properties. This research was performed in order to determine if methanolic extract from the leaves of S. perralderianus had any protective effects on gastroenteritis brought on by alcohol consumption. Wistar rats were fed with 100% ethanol orally to induce gastric ulcer, and pre-treated with 50, 100, and 200 mg·kg-1 of the extract in addition to 5 mg·kg-1 of Ranitidine as a positive reference drug. The extract had shown a positive effect to protect ethanol-induced gastric ulcers with a protection percentage of 71 to 88%. In addition, pretreatment of rats significantly increased levels of GSH, CAT, and SOD in vivo as non-enzymatic and enzymatic antioxidants, and also reduced the level of lipid peroxidation. Histopathological sections, which showed the action of the therapeutic extract, substantially confirmed these findings on the reduction of the inflammation zone and the reduction of immune cell filtration caused by ethanol toxicity with increased extract dosages compared to Ranitidine. The antiulcer activity is due to inhibition of oxidative stress and gastritis. It is associated with a total polyphenol, flavonoids, chlorophyll (a, b), and carotenoids substantial amounts

Citation

Abderrahim BENSLAMA , , (2024-01-06), Preventing ethanol-induced stomach ulcers in rats using Senecio perralderianus leaf extracts, Revista Científica, Vol:34, Issue:1, pages:8, University of Zulia

In recent years, multiple drug resistance in human pathogenic microorganisms has developed due to indiscriminate use of commercial antimicrobial drugs commonly used in treatment of infectious diseases. This situation forced scientists to search for new antimicrobial substances from various sources like medicinal plants which are good sources of novel antimicrobial agents. Flavonoids are ubiquitous in photosynthesising cells and are commonly found in fruit, vegetables, nuts, seeds, stems, flowers, tea, propolis and honey. For centuries, preparations containing these compounds as the principal physiologically active constituents have been used to treat human diseases. Increasingly, this class of natural products is becoming the subject of anti-infective research, and many groups have isolated and identified the structures of flavonoids possessing antifungal, antiviral and antibacterial activity. Since these secondary metabolites are synthesized by plants in response to microbial infection, it should not be surprising that they have been found in vitro to be effective antimicrobial substances against a wide array of microorganisms.Also, their antimicrobial activities against some Gram-negative and Gram-positive bacteria have been reported in many papers. In plants, the flavonoids appear to play a defensive role against invading pathogens, including bacteria, fungi and viruses. There is also evidence suggesting that dietary flavonoids can influence gastrointestinal bacterial populations, and there is considerable in vitro data

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Asma Medjahed , Abderrahim BENSLAMA , ,(2023-11-08), Antimicrobial potential of flavonoids,Séminaire National Hybride Biotechnologie Et Biodiversité Microbienne,Université de Khenchela

Medicinal plants have been frequently used to treat a variety of human diseases. Over the last century, the use of vegetation in medication has affected the identity of natural composites. One of the medicinal plants that have pharmaceutical values is Plantago ciliata Desf. This plant is commonly known as dil elkhrouf, Zelfana, annadam. This plant is belonging to the Plantaginaceae, is a traditional Algerian herbaceous plant abundantly distributed in the northeastern region of the Sahara. It has been reported to have numerous ethnopharmacological properties including anti-inflammatory, anti- ulcer, antitussive and anti-diabetic activities. Plantago genus is a vital medicinal plant that has many combinations of secondary metabolites. It has been used for the treatment of certain conditions such as hyperglycemia and high cholesterol levels. Additionally, the WHO has approved its use as a laxative agent. Nevertheless, relatively few studies have been conducted on the plant, including studies related to the genomic resources of the plant. Most Plantago species are perennial forages, lasting for many years, seemingly hairless, have a small root and several upright or curved stems that are equal to or slightly longer than the leaves and lack spaces. Its leaves are oval (the main type) with large veins and serrated ends, complete and relatively long petioles. Some of the Plantago plants are annuals, 5 to 20 cm tall, less stalked, more or less appressed hairy. Leaves resolute, narrowly oblong-lanceolate to linear-lanceolate. Scape thick, vertical or ascending, longer or shorter than the leaves. Spikes inflexible, very condensed, barely cylindrical, 3-10 cm. Fruit capsule, 3-5 seeds. The utilization of medicinal plants and their derivatives in treating illnesses is more appropriately recognized as herbal remedy than traditional medicine. For centuries, medicinal herbs have been used for the treatment of diseases in many countries.

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Asma Medjahed , Abdallah Rahali , Abderrahim BENSLAMA , ,(2023-10-19), description, traditional uses and biological activities of saharen plantago,3ème édition du Séminaire National Sciences d'Interfaces Chimie-Biologie (hybride).,Universite de Souk Ahras

Moringa oleifera, native to India, grows in the tropical and subtropical regions of the world such Africa.It is commonly known as ‘drumstick tree’ or ‘horseradish tree’. Moringa oleifera belonging to the family of Moringaceae is an effective remedy for malnutrition. Every part of this tree: Seeds, leaves, oil, sap, bark, roots, and flowers are widely used in traditional medicine. Moringa leaves have been characterized to contain a desirable nutritional balance, containing vitamins, minerals, amino acids, and fatty acids. Additionally, the leaves are reported to contain various types of antioxidant compounds such as ascorbic acid, flavonoids, phenolics, and carotenoids. Various preparations of M. oleifera are used for their antiinflammatory, antihypertensive, diuretic, antimicrobial, antioxidant, antidiabetic, antihyperlipidemic, antineoplastic, antipyretic, antiulcer, cardioprotectant, and hepatoprotectant activities. It also has been used to treat skin infections, anemia, anxiety, asthma, blackheads, blood impurities, bronchitis, catarrh, chest congestion, cholera and many other illnesses. This review explores the use of moringa across disciplines for its medicinal value and deals with cultivation, nutrition, and prominent pharmacological properties of this “Miracle Tree”.

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Asma Medjahed , Abdallah Rahali , Abderrahim BENSLAMA , ,(2023-10-03), phytochemistry,ethnobotany,cultivation and pharmacology of moringa oleifera,Séminaire National sur : Biodiversité, Santé, Environnement : Enjeux du Développement Durable,Université de Mila

Background: Ruta montana (R. montana) is a medicinal plant with a long history of traditional use in treating ailments. Objective: The present work aims to study the phytochemical composition and to evaluate the biological activities of R. montana L. (Rutacea) extracts. Methods: Bioactive compounds were obtained using solid-liquid extraction using solvents of increasing polarity. The obtained extracts were qualitatively analyzed by liquid chromatography coupled with mass spectrometry (LC-MS). The pharmacological properties of R. montana were also investigated. Antioxidant activity was achieved "in vitro" using two methods: scavenging of the free radical DPPH and total antioxidant capacity. Antimicrobial activity was evaluated using disc diffusion method on 3 pathogenic bacterial strains (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus). In addition, cytotoxic activity was determined by the "Brine shrimp" test. Results: Chemical investigation allowed the identification of 14 phenolic compounds. The identified compounds were mainly phenolic acids, coumarins and flavonoids. The crude extract and the different tested fractions exhibited an interesting antioxidant activity. Chloroform extract was effective against the growth of the tested bacterial strains with zones of inhibition varying between 14 and 18 mm. In contrast, ethyl acetate and butanolic extracts were almost inactive on all of the tested bacterial strains. Furthermore, the crude extract was found to exhibit antibacterial activity with 11 to 11.66 mm of inhibitions zone. No cytotoxic effect was recorded for all the tested extracts up to a concentration of 4000 μg/mL. Conclusion: This work highlights the potent bioactivity and acceptable drug-likeness of this plant, which supports its further uses.

Citation

Abderrahim BENSLAMA , , (2023-07-17), Phytochemical Profiling, LC-MS Analyses, Cytotoxicity, Antioxidant, and Antimicrobial Activities of Ruta montana: An Ethnomedicinally Important Plant in Algeria, Current Bioactive Compounds, Vol:18, Issue:7, pages:8, benthem

Nowadays, coffee (Coffea Arabica L.) is among the most significant agricultural products of the world and drinking coffee has become one of the most popular habits in the world. The main contamination of stored coffee beans is related with the mycotoxin produced by the toxigenic fungi belonging the genus Aspergillus. Fungal infection followed by mycotoxin biosynthesis in coffee results in notable financial losses. subsequent mycotoxin biosynthesis in coffee leads to major economic losses. Complications ranging from mild to severe can be caused by the mycotoxins produced by this genus. The aim of this investigation was to determine the effect of menthol and eugenol on Aspergillus parasiticus (CBS 100926T) growth, spore germination, and their potential use as green coffee beans preservative during long-term storage (12 months). The minimum inhibitory concentrations (MICs) values of the menthol and eugenol were recorded to completely inhibit the growth of A. parasiticus in 400 μg/ml and 300 μg/ml, respectively. Both reduced spore germination by 9.33% and 5.66% at 300 μg/ml and 200 μg/ml, respectively. They showed efficacy in fumigated green coffee beans sample during the storage for up to 12 months providing an increase in the protection level of 62.5% for menthol and 73.21% for eugenol against the A. parasiticus contamination. This suggests that menthol and eugenol could be used as good alternatives for decreasing the deteriorations due to the fungal infections in green coffee beans during long-term storage.

Citation

Abderrahim BENSLAMA , , (2023-07-01), Antifungal effects of selected menthol and eugenol in vapors on green coffee beans during long-term storage, Heliyon, Vol:9, Issue:7, pages:8, sciencedirect

Medicinal plants have played an important influence in the development of human society; they were always at the forefront of all cultures and civilizations as a source of medicine. Medicinal plants are considered rich sources of secondary compounds, and many modern medications are derived from them. Santolina is the most diverse genus within the mint family (Asteraceae), that is widely distributed in the Mediterranean basin and many of its species are well-known due to their medicinal and culinary uses. The present research discusses the traditional uses, phytochemical components and biological activities of Santolina rosmarinifolia. To do so, we carried out a comprehensive review of the articles available in different online databases published from the past to 2022 (i.e., PubMed, Scopus, and Web of Science, among others). S. rosmarinifolia has several traditional uses, including as anti-inflammatory and an antimicrobial agent, among several others, ascribed to it. In Spain, S. rosmarinifolia’s flowerheads are traditionally used as antipyretic, anti-inflammatory, antihypertensive and hepatoprotective. In Algeria, this plant is used for the treatment of dermatoses. The same uses are also reported in Portugal. The phytochemical studies have revealed the presence of terpenes, including monoterpenes, eudesmane-type sesquiterpenes, germacrene-type sesquiterpenes, dammarane-type triterpenes, coumarins, and flavonoids as the main classes of constituents. A study carried out on santolina Essential oil showed existence of sabinene, β-pinene, myrcene, β-phellandrene, limonene, 1,8-cineole, artemisia ketone, terpinen-4-ol, capillene, ar-curcumene and β-eudesmol. Monoterpenes like β- phellandrene, limonene and 1,8-cineole are also identified. In conclusion, this studys provides much information about the interests of vegetal species: Santolina rosmarinifolia.

Citation

Asma Medjahed , Abderrahim BENSLAMA , ,(2023-06-17), Ethnopharmacology, Phytochemistry, and Pharmacology of Santolina rosmarinifolia: A review,Séminaire National sur : Biodiversité, Santé et Environnement,Université de Temcen

Medicinal plants are considered rich sources of secondary compounds, and many modern medications are derived from them. Santolina is the most diverse genus within the mint family (Asteraceae), that is widely distributed in the Mediterranean basin and many of its species are well-known due to their medicinal and culinary uses. The present research discusses the traditional uses, phytochemical components and biological activities of Santolina rosmarinifolia. To do so, we carried out a comprehensive review of the articles available in different online databases published from the past to 2022 (i.e., PubMed, Scopus, and Web of Science, among others). S. rosmarinifolia has several traditional uses, including as anti-inflammatory and an antimicrobial agent, among several others, ascribed to it. In Spain, S. rosmarinifolia’s flowerheads are traditionally used as antipyretic, anti-inflammatory, antihypertensive and hepatoprotective. In Algeria, this plant is used for the treatment of dermatoses. The same uses are also reported in Portugal. The phytochemical studies have revealed the presence of terpenes, including monoterpenes, eudesmane-type sesquiterpenes, germacrene-type sesquiterpenes, dammarane-type triterpenes, coumarins, and flavonoids as the main classes of constituents. A study carried out on santolina Essential oil showed existence of sabinene, β-pinene, myrcene, β-phellandrene, limonene, 1,8-cineole, artemisia ketone, terpinen-4-ol, capillene, ar-curcumene and β-eudesmol. Monoterpenes like β- phellandrene, limonene and 1,8-cineole are also identified. In conclusion, this studies provides much information about the interests of vegetal species: Santolina rosmarinifolia.

Citation

Asma Medjahed , Abdallah Rahali , Abderrahim BENSLAMA , ,(2023-06-17), ethnopharmacology, and parmacology of santolinaros marinifolia,1er séminaire national sur la valorisation des matières résiduelles du secteur agro-alimentaire et application en santé et maladies,Universite de Tlemcen

Algerian medicinal plants : Traditional Uses, Phytochemistry and Pharmacological Activities

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Abderrahim BENSLAMA , ,(2023-05-26), Algerian medicinal plants : Traditional Uses, Phytochemistry and Pharmacological Activities,Current Drug Research Topics in Pharmacy: Traditional Medicine Talks,Turquie

The extraction of plant materials can be done by various extraction procedures. Non- conventional methods, which are more environmentally friendly due to reduced use of synthetic and organic chemicals, reduced operating time, and higher yield and quality of extract, have been developed over the past 50 years. A search carried out on the PubMed database using the following keywords: supercritical fluid extraction, lavender. This research showed seven studies out of 415 have begun to study the biological activities of lavender, using the method of supercritical fluid extraction and ultrasound-assisted extraction. The result shows that the effect of temperature and pressure on the extraction yield is highly pronounced. To be the optimum conditions to achieve the maximum yield and maximum polyphenols content in scCO2 extract from Lavandula. Further advantages of supercritical fluid extraction are the lack of oxygen which prevents unfavorable oxidation processes and the ease of separating the extractant from the product. Besides, the extracts obtained with supercritical fluids and ultrasound-assisted extraction showed a higher antimicrobial and DPPH scavenging activities compared with maceration extracts. This improved final product with the application of green technology could have potential applications in food, cosmetic and pharmaceutical industries as a preservative or nutraceutical.

Citation

Asma Medjahed , Abdallah Rahali , Abderrahim BENSLAMA , ,(2023-05-13), Non-conventional techniques for bioactive compounds extraction from lavender:,1er Webinaire National sur la Chimie Verte dans la Protection de l’Environnement et le Développement Durable,Universite de Laghouat

Les molécules à usage anti-inflammatoire commercialisées en Algérie au jour d'aujourd'hui, à l'instar de l'ibuprofène, causent bon nombre de pathologies en raison de leurs nombreux effets secondaires à posologie usuelle et en administration percutanée (complications cardio-pulmonaires, hypertension artérielle, insuffisance rénale). L'Algérie de part sa grande superficie et ses variations climatiques, donne lieu à une variabilité et une diversité des espèces végétales. Ajoutant à cela que notre pays riche de son patrimoine traditions, nous offre un éventail important et différent de l'usage médicinal fait de ces plantes. Les molécules bioactives issues de ces plantes, suscitent actuellement un intérêt grandissant dans les domaines pharmaceutique. Le regain d’intérêt en thérapeutique pour ces biomolécules est motivé par le fait qu’elles présentent des effets secondaires limités ou inexistants comparativement aux molécules synthétiques qui en plus de causer de nombreux effets secondaires indésirables présentent des limites thérapeutiques. Notre étude, qui s’inscrit dans l’optique de valorisation des ressources végétales, a pour objectif, l’évaluation du potentiel anti-inflammatoire de quelques plantes médicinales de Kabylie (carthamus caeruleus, Rosa sempervirens, Lavandula stoechas, Romarinus officinalis, Malva sylvestris, Arbutus unedo). L’appréciation de la capacité anti-inflammatoire des plantes étudiées, a été effectué par le test de stabilisateur des membranes érythrocytaires vis-à-vis de différents stress (osmotique, oxydant induit par le HOCL et la chaleur) et par la stabilisation thermique de la sérum albumine. La somme des résultats obtenus au cours de cette étude montre clairement que les extraits aqueux de feuilles des plantes étudiées, possèdent un potentiel anti-inflammatoire important. De ce fait, ces plantes constituent une source potentielle de molécules bioactives et ainsi constituent une alternative thérapeutique au traitement de nombreuses pathologies initiées par le stress oxydant.

Citation

Abderrahim BENSLAMA , Mk idir, Beunis yussra, ,(2023-05-04), Activité anti-inflammatoire de quelques plantes médicinales de Kabylie,International Seminar on Bioresources and Sustainable Development,Université de Bouira

Hertia cheirifolia L. is a medicinal plant belonging to Asteraceae family. It is traditionally used to treat gastrointestinal ailments, infections, and spasms. The aim of this study is to evaluate the oral acute toxicity of aqueous extract from Hertia cheirifolia on female Albino wistar mice. Animals were divided into three groups, the first group received distilled water and was used as control; Mice of the second and third groups received the plant extract at doses of 2g/kg and 5g/kg body weight of animals, respectively. The mice were monitored for 14 days for their general behavior, adverse effects and mortality. The results showed that no death or toxic signs were observed, no changes were seen in food consumption, body weight and organ weight in the treated animals compared to the controls. However, an increase in AST and ALT levels was recorded in mice treated with the extract.

Citation

Abderrahim BENSLAMA , , (2023-04-24), Acute Toxicity Evaluation of Aqueous Extract from Hertia cheirifolia in Swiss Albino Mice, AMA, Agricultural Mechanization in Asia, Africa and Latin America, Vol:54, Issue:4, pages:7, Shin-Norinsha Co

Centaurea parviflora (C. parviflora), belonging to the family Asteraceae, is an Algerian medicinal plant used in folk medicine to treat different diseases related to hyperglycemic and inflammatory disorders, as well as in food. The present study aimed to assess the total phenolic content, in vitro antioxidant and antimicrobial activity and phytochemical profile of the extracts of C. parviflora. The extraction of phenolic compounds from aerial parts was conducted using solvents of increasing polarity starting from methanol, resulting in crude extract (CE), to chloroform extract (CHE), ethyl acetate extract (EAE) and butanol extract (BUE). The total phenolic, flavonoid and flavonol contents of the extracts were determined using the Folin–Ciocalteu and AlCl3 methods, respectively. The antioxidant activity was measured with seven methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, galvinoxyl free-radical-scavenging test, 2,2′-Azino-Bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) assay, cupric reducing antioxidant capacity (CUPRAC), reducing power, Fe+2-phenanthroline reduction assay and superoxide-scavenging test. The disc-diffusion method aimed at testing the sensitivity of bacterial strains toward our extracts. A qualitative analysis with thin-layer chromatography of the methanolic extract was performed. Moreover, HPLC-DAD-MS was used to establish the phytochemical profile of the BUE. The BUE was found to contain high amounts of total phenolics (175.27 ± 2.79 µg GAE/mg E), flavonoids (59.89 ± 0.91 µg QE/mg E) and flavonols (47.30 ± 0.51 µg RE/mg E). Using TLC, different components such as flavonoids and polyphenols were noted

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Abderrahim BENSLAMA , Fatima Zohra Hechaichi, Hamdi Bendif, Chawki Bensouici, Sulaiman A Alsalamah, Boutheina Zaidi, Mustapha Mounir Bouhenna, Nabila Souilah, Mohammed I Alghonaim, Abderrahim Benslama, Samir Medjekal, Ashraf A Qurtam, Mohamed Djamel Miara, Fehmi Boufahja, , (2023-02-28), Phytochemicals, Antioxidant and Antimicrobial Potentials and LC-MS Analysis of Centaurea parviflora Desf. Extracts, Molecules, Vol:28, Issue:5, pages:28, MDPI

Phytochemical analysis, Antibacterial effect and the antioxidant activity of Atriplex halumus extracts

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Abderrahim BENSLAMA , ,(2022), Phytochemical analysis, Antibacterial effect and the antioxidant activity of Atriplex halumus extracts,International Online Conference on Valuation of Alternative Plants, Degraded & Margin,Université, EL- OUED

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Abderrahim BENSLAMA , ,(2022), Activité anti-inflammatoire et antioxydante de citron (Citrus limon),Journées scientifique Biodiversité , santé et environnement,Université de Bouira

Isolation, identification and antibacterial activity of endophytic fungi from Marrubium vulgare L

Citation

Abderrahim BENSLAMA , ,(2021), Isolation, identification and antibacterial activity of endophytic fungi from Marrubium vulgare L,Séminaire national : Bio-ressources: Nutrition, Santé et Environnement.,Université de M'sila

Background: The use of plants for the treatment, cure and prevention of diseases is one of the oldest medicinal practices of humanity. These plants are able to produce much diversified natural compounds (e.g. alkaloids, polyphenols, etc.) which have many pharmaceutical applications. Objective: The aim of the present study was to evaluate the antioxidant capacity and antibacterial activity of Zizyphus lotus extracts, which has been used traditionally in folk medicine of Algeria. Methods: The leaves of plants were submitted to extraction by water and methanol to give methanolic (Met.E) and aqueous (Aq.E). The extracts were analyzed by HPLC-TOF/MS and their antioxidant activity was determined using DPPH and ABTS free radical-scavenging test, when the antibacterial activity was determined using disc diffusion method. Results: The quantitative analysis of polyphenols and flavonoids content showed that the Met.E had the highest amount (562.04 µg EAG/mg E and 66.34 µg EQ/mg E, respectively). The HPLC-TOF/MS analysis of Met.E reveals the presence of two major compounds, rutin and gentisic acid, when Aq.E contain rutin and quercetin-3-β-D-glucoside as a major compound. Results showed that the Met.E exhibited the highest antiradical activity against DPPH and ABTS free radical with EC50=28.19±0.51 µg/mL, and EC50=3.82±0.03 µg/mL, respectively. Moreover, the Met. E of Z. lotus presents a moderate antibacterial activity against Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis, with inhibition zone ranging from 09 to 13 mm. In addition, The Met.E showed a better MIC against M. luteus and B. subtilis (MIC= 25 µg/mL). Conclusion: Our results showed that the methanolic extract of Z. lotus reported a considerable antioxidant and antibacterial activities which may be due to their richness on polyphenols and flavonoids. Keywords: Antioxidant, antibacterial, Z. lotus, phenolic compounds, HPLC-TOF/MS, disc diffusion method, methanolic (Met.E) extract.

Citation

Abderrahim BENSLAMA , Abdenassar HARRAR , Fatih GÜL, Ibrahim DEMİRTAŞ, , (2017), Phenolic Compounds, Antioxidant and Antibacterial Activities of Zizyphus lotus L. Leaves Extracts, The Natural Products Journal, Vol:7, Issue:4, pages:1-7, Bentham science

Ethnopharmacological relevance: Reichardia picroide is a species mainly used for alimentary purposes, but it is traditionally known to be used as hypoglycemiant, diuretic, depurative, galactagogue and tonic. Aim of the study: To date, there are no studies corroborating both its safety and antioxidant activities. The objective of the present study, thus, was to assess the safety profile of Reichardia picroide methanolic extract (RPE) and as well as on its antioxidant and antihemolytic activities. Materials and methods: The acute toxicity of RPE was carried out based on OECD guidelines 425. Signs accompanying toxicity and possible death of animals were monitored for two weeks to ascertain the medianlethal dose (LD50) of the RPE. In sub-acute toxicity study, the extract was administered by gavage at the doses of 250, 500 and 1000 mg/kg/day for 21 consecutive days. The antioxidant activity of RPE was investigated through various methods both in vitro and in vivo. Results: The admistrated doses did not produce mortality or changes in general behaviors of the tested males and females mice. The LD50 was found to be superior to 5000 mg/kg DW. Moreover, daily administration of RPE at doses ranged from 500 to 1000 mg/kg could result in alteration of liver and kidney histology. Significant decrease in liver enzymes (ALT and AST), urea and creatinine levels in female plasma was recorded. The RPE was, in vitro, strong in DPPH scavenging and hemolytic inhibition, benificial in lipid peroxidation inhibition and reducing power. In addition, it exhibited, in vivo, a strong effect on GSH level increasing and lipid peroxidation inhibition in liver and kidney. Conclusions: It can be suggested, based on the results of this study, that the crude extract of Reichardia picroide was non-toxic in acute administration and the use of this extract is safe at doses ≤ 250 mg/kg. This study supports the application of Reichardia picroides in alimentary and traditional medicine purposes. Moreover, antioxidant activity results suggested that Reichardia picroide had potent antioxidant activities and could be utilized as new natural antioxidant in food and therapeutics.

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Abderrahim BENSLAMA , Sana AOUACHRIA, Sabah BOUMERFEG, Fayçal BENBACHA, Thorya GUEMMAZ, , (2017), Acute, sub-acute toxicity and antioxidant activities (in vitro and in vivo) of Reichardia picroide crude extract, Journal of Ethnopharmacology, Vol:2017, Issue:208, pages:105-116., Elsevier

The aim of this study is to evaluate antioxidant activity of aqueous (Aq.E) and methanolic (Met.E) extracts of Zygophyllum album L. (Zygophyllaceae) and Arthrophytum scoparium (Chenpodiacea), two medicinal plant from Algerian Sahara used in traditional pharmacopoeia. The results showed that Aq.E have the heights yields 24% and 22.3% for Z. album and A. scoparium respectively. Qualitative analysis of showed that the Met.E of Z. album of had the highest amount of polyphenols (202.26±4.73 µg GAE/mg E) and flavonoids (15.77±0.94 µg QE/mg E). The antioxidant activity of extracts was evaluated by 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay. Results showed that the extracts of A. scoparium. Have the highest free radical scavenger activity in DPPH test (EC50=0.096±0.001 and 0.108±0.002 mg/ml for Met.E and Aq.E respectively). In addition, the results of reducing power assay demonstrated that Met.E of Z. album had the highest effect with 2399.65±12.31 µg ascorbic acid equivalent/mg E.Our results showed that the methanolic extract reported a considerable free radicals scavenging activity and reducing effect, may be due to their richness on polyphenols and flavonoids.

Citation

Abderrahim BENSLAMA , abdenassar.harrara@univ-msila.dz, , (2016), Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts., International Journal of Herbal Medicine, Vol:4, Issue:6, pages:158-161, Akhil Gupta

This work was carried out to study the antioxidant capacity and antibacterial activity of Limoniastrum guyonianum (Plombaginaceae) extracts, traditionally used in folk medicine in Algeria. The aerial part were submitted to extraction by water and methanol to give methanolic (Met.E) and aqueous (Aq.E) extracts with that yields are 10%and 17,5% respectively. Quantitative analysis of polyphenols and flavonoids content showed that the Met. E had the highest amount (285.37µg EAG/mgE and 44.11µg EQ/mg E, respectively). The antioxidant activity of extracts was evaluated by 2,2’-diphenyl-1-picrylhydrazyl (DPPH) and ABTS free radical-scavenging test. Results showed that the Met. E exhibited the highest antiradical activity against DPPH and ABTS free radical with EC50=0,099 mg/ml, and EC50=0.024mg/ml, respectively. Moreover, the antibacterial activity was determined on the five bacterial strains, using disk diffusion method. The Met. E showed inhibition zone averages only on M. luteus, and S. aureus (12 and 13 mm respectively) and with MIC is 25 μg/ml. Our results showed that the methanolic extract of L. guyonianum reported a considerable antioxidant and antibacterial activities which may be due to their richness on polyphenols and flavonoids.

Citation

Abderrahim BENSLAMA , Abdenassar HARRAR , Safia AFISSA, Asma SAIDI, , (2016), Total phenolic contents, antioxidant and antibacterial activities of Limoniastrum guyonianum (Plombaginaceae) extracts, International Journal of Current Research, Vol:2016, Issue:812, pages:42675-42679, akhil kapio