LAID Benderradji

CHBIRA Salima , CHAREF Abdellatif

Test de germination in vitro et régénération des plantules sous stress salin ches le quinoa (Chenopodium quinoa Willd)

TRIRAT Chaima , LAABIDI Bouchra

Contribution à l'étude des plantes médicinales et leurs utilisations thérapeutiques dans la daira de Ras El Oued et Bordj Ghedir dans la Wilaya de B. B. A.

Benabderrazak Bouzid , Hedjouli moussa

Germination et caractérisation morphologique sous stress salin et hydrique chez deux céréales à paille (Avena sativa L.) et (Hordeum vulgare L.)

Attri Bilal , Attar Naima, Laachache Bassma

Transformation technologique de blé tendre (Triticum aestivum L.) : Analyses physico-chimiques et caractérisation de la farine panifiable

Mimoune Oumayma

Quinoa (Chenopodium quinoa Willd) : Caractérisation chimique, intéret nutritionnel et propriétés pharmacologiques

Description du sujet : Proteases represented 60–65% of the total industrial enzyme market, most of which are alkaline proteases. Most of these find applications in the food industry, in the meat tenderization process, peptide synthesis, for infant formula preparations, detergent formulations, baking and brewing. Objectifs : This study aim was alkaline protease characterization and production from local bacterial strain Halobacillus salinus DZ28. Méthode: In this subject, a new Algerian Gram-positive, rod-shaped, endospore-forming, salt-philic bacteria (DZ28 strain) that overproduce extracellular alkaline proteases have been isolated from salt lake deposits in Lake Oubeira, El Taref. Strain DZ28 was assigned as Halobacillus salinus DZ28 on the basis of phenotypic properties and 16S rDNA gene sequencing (ripotyping). Résultats et discussion : The maximum protease activity registered after 36 hours of incubation in optimized medium at 30° C was 19,000 U / ml in a shaking bottle culture at 160 rpm. The crude extract protease showed optimal activity at 60 ° C temperature and pH 12. It is actively inhibited by PMSF and DIFP, indicating that it belongs to the serine protease family. Interestingly, the crude extract protease was not only very stable to nonionic surfactants and oxidants, but also showed high stability and compatibility with some commercial detergents. It retaining more than 100% of its initial activity after pre-incubation for 1 h at 40°C with ISIS, followed by Pril (98%), Tide (95%) and Dixan (90%). Interestingly, the performance evaluation of the wash revealed that blood stains can be effectively eliminated at a temperature of 40°C for 1 hour with minimal supplementation (500 U/mL). Conclusion : This study represents the initial report of a protease derived from Halobacillus salinus, indicating its potential as a highly promising bioadditive for future applications in detergent formulations. Mots clés : Halobacillus salinus, Protease, detergent, Wash performance.

Citation

Samir MEDJEKAL , MOULOUD Ghadbane , Laid BENDERRADJI , ,(2025-04-15), Identification of a novel protease from Halobacillus salinus strain DZ28 isolated from salt lake sediments in Algeria and their use as detergent bioadditives,Le 1er Séminaire national en Santé et Sécurité sanitaire des aliments,Université Mohamed boudiaf-M'sila