AHLAM Guettouchi

Ketfi salma , Guergueb hadjer, Mansouri roumaissa

Biotechnologies et l'effet du stress hydrique sur le mais (Zea mays L).

Tharafi aycha , Ziani ahlam, Mazouzi ilham

Biotechnologies et l'effet du stress hydrique sur le palmier dattier (Phoenix dactylifera L.)

Loubazid fatna , Foudili salma

Contribution à la culture in vitro d'amandier

Zoubiri halima saadia , Khaouni chaima, Ghalem ouissem

La culture in vitro de palmier dattier (Phoenix dactylifera L.) Avantages et inconvénients

Menasri Zahra , Khodja Rayhana

Les étapes de la culture in vitro du palmier dattier (Phoenix dactylifera L.).

Saoud Dounya , Rahli Meriem

La culture in vitro du palmier dattier (Phoenix dactylifera L.).

Bennaceur Warda , Louchani Nour Elhouda.

Caractérisation cytogénétique de palmier dattier (Phoenix Dactylifera L.).

Rahali Amel , Khelifi Fatima Zahra.

Biodiversité et multiplication in vitro du figuier (Ficus carica L.)

Bouras Hanane.

Biodiversité et multiplication in vitro du grenadier (Punica granatum).

Oukérimi Khiera , Oucif asma.

La biodiversité des arbres fruitiers dans la région de M’sila

Achouri. F. , Bisker .S.

Les méthodes de régénération du palmier dattier (Phoenix dactylifera L.)

The Oasis of Bou-Saada is gradually deteriorating due to the attack of insect pests such as white scale Parlatoria blanchardi Targ., the palm worm Myeloïs ceratoniae Zell and some fungal pests. In addition, polluted irrigation water coming from the wadi Bou-Saada has seriously damaged the oasis palm. Consequently, 23 promising date palm varieties are facing extinction. The Rehabilitation of the oasis palms of Bou-Saâda requires mass propagation of the endangered date palm varieties to conserve the fauna and the genotypes. The Tissue Culture technique is the only method through which date palm oases Bou-Saâda can be restored in a short period as compared to growing the trees through seeds or offshoots. As the first phase of conservation of the oases, this research project was initiated whereas propagation of two highly promising varieties Deglet-Nour and Mech-Degla was carried out through tissue culture technique on MS medium using various concentrations of three different hormones (AIB, GA3, 2,4-D) to obtain organogenesis. Results revealed that the growth in length of explants of the variety Deglet-Nour and its overall development was better as compared to the variety Mech-Degla with the AIB (2mg/l). The GA3 hormone performed the best in relation to the development of organogenesis in the two varieties followed by AIB hormone than the 2,4-D with overall mean lengths of 1.36, 1,26 and 0.84 centimetres, respectively. The formation of the callus was noticed only in the case of the variety Deglet-Nour. Key words: Bou-Saâda oasis, In vitro culture, Deglet-Nour, Mech-Degla, 2,4-D, AIB, GA3, Organogenesis.

Citation

Ahlam GUETTOUCHI , ADMIN Admin , , (2022-07-20), THE EFFECT OF SOME HORMONES ON THE IN VITRO CULTURE OF DATE PALM (PHOENIX DACTYLIFERA L.) OF BOU-SAÂDA, ALGERIA, Pakistan Journal of Botany, Vol:546, Issue:1, pages:2089 - 2093, Pakistan Botanical Society.

Molecular markers are a good way to study genetic diversity in plants, which play importance role in plant improvement programs. In date palm (Phoenix dactylifera L.), many molecular markers (RAPD, ISSR, SSR…) have been used for several purposes. The most important of which is the molecular identification of date palm varieties, the study of the genetic convergence between varieties, to identify resistant varieties to Bayoud disease, to verify the genetic compatibility of the vitro-plants obtained from tissue culture, in addition to these uses it was possible to determine the sex of the date palm by molecular markers. More investigations are needed to answer questions such as: Could molecular markers identify all date palm inheritance? Could molecular markers identify danger palm diseases?

Citation

Ahlam GUETTOUCHI , ,(2018-03-19), Date Palm: Application of molecular markers,Sixth International Date Palm Conference (SIDPC), which will be held in Abu Dhabi during 19 – 21 March, 2018”,Abu Dhabi, United Emirates

In this study we investigated the ISSR and RAPD polymorphisms in date palm cultivars collected from Bou-Saada oasis, located at the bases of the Ouled Nail Range of the Saharan Atlas of Algeria. Both markers resulted in high and comparable levels of polymorphisms; around 75% polymorphic loci were detected among 151 ISSR and 198 RAPD alleles. The contribution of RAPD and ISSR markers to total gene diversity was 0.225 and 0.220 and to Shannon's Information index was 0.344 and 0.337, respectively. At group levels, soft, dry and median types of cultivars characterized by presence of unique alleles to each group noticeable in considerable differences in gene diversity as well as Shannon's Information indices. However, the amount of shared alleles within groups resulted in strong variation (100% for ISSR and 98% for RAPD) explained within groups of individual cultivars rather than among groups. Generally, RAPD and ISSR markers resulted in disparate clustering patterns, however, both markers configured the close relationship between Rotabaya and Ghars cultivars with 84% bootstrap value (BV; RAPD) and 63% BV (ISSR). The cultivar, Baarit- djemal is strongly identified as possible progenitor of the sampled male tree. Baarit-djemal cultivar and the male tree found closely related with BV equaled to 90% for polymorphism of combined markers, 71% with RAPD markers and 50% with ISSR markers.

Citation

Ahlam GUETTOUCHI , , (2017-02-23), MOLECULAR DIVERSITY IN DATE PALM (Phoenix dactylifera L.) CULTIVARS FROM ALGERIA INDICATED BY RAPD AND ISSR POLYMORPHISMS, PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY,, Vol:18, Issue:1, pages:76 - 89, I. K. Press

Bayoud disease of the date palm (Fusarium oxysporum f. sp. albedinis) is a vascular disease caused by a soil fungus. Twenty one Algerian date palm cultivars (20 females and 1 male) in addition to 6 genotypes of Deglet Nour were assessed for resistance (R) and susceptibility (S) to Bayond disease using molecular markers. Specifically, the use of two circular plasmid-like DNAs (R and S plasmids) as molecular markers allowed us to detect the R cultivars. Among the 21 cultivars, we found that Baâret ljmal is resistant.

Citation

Ahlam GUETTOUCHI , , (2017-02-20), Molecular characterization of Algerian date palm cultivars using circular plasmid-like DNAs, IIndian J. Genet, Vol:77, Issue:1, pages:170 - 172, the Indian Society of Genetics & Plant Breedi

The oasis of Bou-Saada,the gate of desert, is one of old traditional Algerian oases with a distance of 250 kilometers from the coast. It is divided into four regions: Djenane Nakhara, Djenane Btom, Djenane Hmaïd, and Djenane Khachbet mimoun with an estimated area of 110 hectares. The declining of the oasis palms is due to several factors, the most important is a number of agricultural pests, which Parlatoria blanchardi Targ. is the most common insect. The highest infestation were 45.58% and 33.1%in Djenane Nakhara and Djenane Hmaïd, respectively. In addition, moth of palm, Myeloïs ceratoniae Zell., where the highest infestation was 34.45% in Djenane Hmaïd. The declining resulted in losses of local varieties, which are important for the region in term of genetics; however, Pollution is contributing to this declining. Our objective is to find out factors that lead to the declining to promote agriculture in the region.

Citation

Ahlam GUETTOUCHI , ,(2016-05-23), DETERMINING FACTORS OF DECLINING OF PALM OASIS OF BOU-SAADA TO PROMOTE AGRICULTURAL SECTOR,CONFERENCE ON THE INVESTMENT IN DATE PALM SECTOR (REALITY AND PROSPECT)At: OMAN,OMAN

L’objectif principal de cette étude est de recenser le nombre de palmiers et de cultivars restants dans l’oasis de Bou-Sâada qui est considérée comme la plus proche Oasis d’Alger et du littoral méditerranéen (250 Km). La palmeraie de Bou-Sâada entre 1850-1860 comptait 10 000 palmiers, il n’en reste que 6000 palmiers environ en 2002. Le nombre de pieds recensé est 2147. Ainsi, la palmeraie s’amenuise d’année en année sous la pression du bâti et du morcellement des exploitations, appelées, « djenane ». Ainsi la superficie de la palmeraie qui s’étendait sur 155 Ha n’en comptait aujourd’hui que 110 ha. Les 23 cultivars ainsi recensés dont la moitié est originaire de Bou-Saâda, sont réparties dans 04 zones, de part et d’autre de l’Oued-Bou-Saâda : Djenane Nakhara, Djenane Btom, Djenane Hmaïd, Djenaneet Khachbet-Mimoun. Les cultivars les plus répandues sont : Bou-Saadiya (35,44%), Halwa (15,18%),DegletNour (9,82%), Rotbaya (8,15%) et Horra (6,28%). La sauvegarde de l’oasis de Bou-Saâda est plus que nécessaire afin de préserver le site et surtout les cultivars menacés par l’urbanisation et l’abandon.

Citation

Ahlam GUETTOUCHI , , (2015-06-15), INVENTAIRE ET CONSERVATION DE LA PALMERAIE DE BOU-SAÂDA, ALGÉRIE, Revue Recherche agronomique, Vol:27, Issue:, pages:48 - 56, Institut National de la Recherche Agronomique d’Algérie

The Bayoud of the date palm (Fusarium oxysporum f. sp. Albedinis), is a vascular disease caused by a soil fungus. It’s considered the most lethal disease of the date palm and, it is a threat to North African date palm plantations as it is widespread in Morocco and Algeria. Two approaches are used to fight this disease; the first approach lies in the search for resistant, high quality cultivars and clones among the local date palm population, while the second approach consists of creating resistant and superior cultivars through a hybridization programme. Recently, the development of DNA markers has offered the possibility of identifying and developing a new approach to improving the efficiency of selection strategies of resistant varieties. In this study we used the molecular markers to identify the resistant (R) and/or the cultivars susceptible (S) to Bayoud; the use of the two circular plasmid-like DNAs (R and S plasmids) as molecular markers allow to detect the R cultivars susceptible to Bayoud. In the first step we used 21 Algerian cultivars of date palm (20 females and 1 male) while in the second step we used 6 genotypes of Deglet Nour. Among the 21 cultivars we found Baâret ljmal is the resistant cultivar (containing the R sequence only) and Deglet Nour represented two plasmids R and S. Using this technique can established Algerian date palm cultivars as R and/or S susceptible to Bayoud.

Citation

Ahlam GUETTOUCHI , ,(2014-03-16), THE APPLICATION OF THE CIRCULAR PLASMID-LIKE DNAs AS MOLECULAR MERKERS FOR THE BAYOUD OF THE DATE PALM (Phoenix dactylifera L.),Fifth International Date Palm Conference 16 - 18 March, 2014.,Abu Dhabi, United Emirates

The Bayoud of date palm (Fusarium oxysporum f. sp. Albedinis), is vascular disease caused by a soil fungus, which considered the most lethal disease of date palm. It is a threat to North African date palm plantations as it is widespread in Morocco and Algeria. Two approaches are the strategy to fight this disease; the first approach lies in the search of resistant, high quality cultivars and clones among local date palm population, while the second approach consists of creating resistant and superior cultivars through a hybridization program. Recently, the development of DNAmarkers offers the possibility to identify and to develop a new approachto improve the efficiency of selection strategies of resistant varieties. In this study we used the molecular markers to identify the resistant (R) and/or the susceptible (S) cultivars to Bayoud; the use of the two circular plasmid-like DNAs (R and S plasmids) as molecular markers allow to detect the R cultivars to Bayoud. In the first step we used 21 Algerian cultivars of date palm (20 females and 1 male) while in the second step we used 6 genotypes of Deglet Nour. Among the 21 cultivars we found Baâret ljmal is the resistant cultivar (contains the R sequence only) and Deglet Nour represented two plasmids R and S. Using this techniquecan established Algerian date palm cultivars as R and/or S to Bayoud.

Citation

Ahlam GUETTOUCHI , ,(2013-11-03), THE APPLICATION OF THE CIRCULAR PLASMID-LIKE DNAs AS MOLECULAR MERKERS FOR THE BAYOUD OF THE DATE PALM (Phoenix dactylifera L.),The Fifth Symposium on Date Palm in Saudi Arabia (Biotechnology in Date Palm),King Faisal University Alahsa, Kingdom of Saudi Arabia 3 - 5 November, 2013

The objective of our work is to study the answer of was to study the response of five vine variéties ( Vitis vinefera L.) to the in vitro culture. The design employed counts two basic mediums, MS and NN, complemented with various hormones. Explant response was measured through leaf emission and leaf extension, roots emission and the ability to acclimation. Results showed that MS was globally more efficient as growth medium as far as genotype response was concerned. Certain varieties exhibited a specific behavior toward a medium or the other for the measured variables. Only one variety, the 140 R showed a large adaptation to mediums and hormones used for the initiation of callus, morphogenesis, rhizogenesis and acclamation. Keywords: Vitis vinefera L –hormones – morphogenesis – rhizogenesis – acclamation.

Citation

Ahlam GUETTOUCHI , , (2011-12-14), ETUDE COMPARATIVE DE LA REPONSE DE CINQ VARIETES PORTES GREFFES DEVIGNE ( Vitis vinifera L.) A LA CULTURE IN VITRO, Sciences & Technologie C, Vol:34, Issue:, pages:40 - 47, Université Mentouri Constantine, Algérie

تبعد واحة بوسعادة عن العاصمة الجزائر بـ 250 كلم التي تعتبر أقرب واحة إلى العاصمة وساحل البحر الأبيض المتوسط. تم اختيار هذه الواحة للدراسة نظرا لأهمية موقعها الجغرافي المميز وجمالها الطبيعي حيث بلغ عدد النخيل فيها 10000 نخلة بين عامي 1850 - 1860م. أما في عام 2002م فلم يبقى ألاّ حوالي 6000 نخلة وهي في تناقص مستمر بالإضافة إلى نقص المساحة التي كانت تحتلها حيث أصبحت 110 هكتار بعد أن كانت 155 هكتار. هذه الدراسة اعتمدت على دراسة الوضعية الحالية للواحة مع إحصاء الأصناف المتواجدة بها ومعرفة أسباب التدهور للنخيل وإعطاء الحلول الممكنة. ينحصر النخيل بصفة عامة في أربعة مناطق هي: جنان نخارة، جنان البطم، جنان الحمايد وجنان خشبة ميمون. كما أن هناك تنوع هام في أصناف النخيل التي بلغت أكثر من 20 صنف مع تواجد أصناف محلية لا توجد إلاّ في هذه الواحة مثل: بوسعادية ونبقايا. ويمثل صنف بوسعادية أهم الأصناف حيث تقارب زراعته 50 % مثل ما هو عليه في جنان نخارة. الهدف من هذه الدراسة هو حماية هذه الواحة والمحافظة على أصناف النخيل بها كمصدر وراثي هام لهذه الواحة.

Citation

Ahlam GUETTOUCHI , كمال شريف ، نادية خلف ، فاطمة عبد الكريم ، هاجيرة قادري ، فاطمة الزهراء ، مديحة مهدي ، هبة سلطاني, ,(2011-12-04), واحة بوسعادة : دراسة الوضعية الحالية وحصر أصناف نخيل التمر,المؤتمر العلمي الأول لتنمية قطاع النخيل والتمور في الوطن العربي,مدينة الملك عبد العزيز للعلوم و التقنية ،الرياض ، المملكة العربيةالسعودية

تحتل زراعة النخيل موقعا مهماً في القطاع الزراعي الجزائري حيث تعتبر المصدر الرئيسي لـ 2,2 مليون نسمة وزراعتها موزعة على 17 ولاية بمساحة إجمالية تقدر بـ 120830 هكتار خلال عامي (2001 – 2002م) بزيادة تقارب من 15,7% مقارنتاً بعامي (2000 – 2001م)، أي بمعدل 112 نخلة/هكتار. نجد ما بين 13,5 مليون نخلة حوالي 69,4% منتجة للتمر. تعتبر ولايات الوادي، بسكرة، أدرار وورقلة من أهم الولايات المنتجة للتمر حيث يقدر إنتاجها بـ 87,8% من الإنتاج الإجمالي للجزائر بينما 58,14% من الإنتاج الوطني في ولايتي الوادي 29,50% وبسكرة 28,6%. أن أهم صنف من أصناف التمور بالجزائر هي دقلة نور التي تمثل 48,3 %من الإنتاج الوطني حيث أنها تزرع في 10 ولايات وتعتبر ولايات الوادي، بسكرة، ورقلة وغرداية من الولايات المهمة لزراعتها حيث تنتج ما نسبته 99%. ويلي الأهمية بعد صنف دقلة نور التمور الجافة بنسبة 30,5 %. وتعتبر ولايتي أدرار و بسكرة من أهم الولايات للتمور الجافة حيث تنتج ما يقارب من %64. وبرغم التطور في مجال إنتاج النخيل إلا أنه يحتاج إلى المزيد من الدراسات والأبحاث لتطوير هذا القطاع الهام اقتصادياً للجزائر.

Citation

Ahlam GUETTOUCHI , ,(2009-12-09), التحدي والنظرة المستقبلية لوضع قطاع النخيل في الجزائر,اللقاء العلمي الأول حول للنخيل و التمور في فلسطين,فلسطين فيديو كونفيرنس) جامعة الاقصى)

تحتل زراعة النخيل موقعا مهماً في القطاع الزراعي الجزائري حيث تعتبر المصدر الرئيسي لـ 2,2 مليون نسمة وزراعتها موزعة على 17 ولاية بمساحة إجمالية تقدر بـ 120830 هكتار خلال عامي (2001 – 2002م) بزيادة تقارب من 15,7% مقارنتاً بعامي (2000 – 2001م)، أي بمعدل 112 نخلة/هكتار. نجد ما بين 13,5 مليون نخلة حوالي 69,4% منتجة للتمر. تعتبر ولايات الوادي، بسكرة، أدرار وورقلة من أهم الولايات المنتجة للتمر حيث يقدر إنتاجها بـ 87,8% من الإنتاج الإجمالي للجزائر بينما 58,14% من الإنتاج الوطني في ولايتي الوادي 29,50% وبسكرة 28,6%. أن أهم صنف من أصناف التمور بالجزائر هي دقلة نور التي تمثل 48,3 %من الإنتاج الوطني حيث أنها تزرع في 10 ولايات وتعتبر ولايات الوادي، بسكرة، ورقلة وغرداية من الولايات المهمة لزراعتها حيث تنتج ما نسبته 99%. ويلي الأهمية بعد صنف دقلة نور التمور الجافة بنسبة 30,5 %. وتعتبر ولايتي أدرار و بسكرة من أهم الولايات للتمور الجافة حيث تنتج ما يقارب من %64. وبرغم التطور في مجال إنتاج النخيل إلا أنه يحتاج إلى المزيد من الدراسات والأبحاث لتطوير هذا القطاع الهام اقتصادياً للجزائر.

Citation

Ahlam GUETTOUCHI , ,(2007-05-05), التحدي والنظرة المستقبلية لوضع قطاع النخيل في الجزائر,The Fourth Symposium on Date Palm in Saudi Arabia,Date Palm Research Center King Faisal University Hofuf, Kingdom of Saudi Arabia