MOHAMMED Allouani
محمد علواني
mohammed.allouani@univ-msila.dz
0668457396
- Departement of Microbiology and biochemistry
- Faculty of Sciences
- Grade PHd
About Me
Sciences de la Nature et de la Vie
Location
Msila, Msila
Msila, ALGERIA
Code RFIDE- 1995-07-08 00:00:00
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MOHAMMED Allouani birthday
- 2025-09-30
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2025-09-30
Otospermum glabrum extracts: antioxidant properties and bioactivity against fungal pathogens and Aphis fabae
The adoption of plant-based biopesticides as sustainable alternatives to synthetic chemicals in agricultural pest management has gained increasing attention due to their safety and ecological benefits. Many of these natural compounds demonstrate potent antifungal activity, mitigating foodborne fungal contamination and associated mycotoxins. This study evaluated the antioxidant, antifungal, and aphicidal potential of Otospermum glabrum (Asteraceae) extracts derived from its aerial parts. Three extracts −ethyl acetate, methanolic, and aqueous− were assessed for total polyphenolic and flavonoid contents, antioxidant activity (DPPH radical scavenging, β-carotene bleaching, and total antioxidant capacity), antifungal effects against six post-harvest and crop fungal pathogens, and aphicidal activity against the black bean aphid (Aphis fabae Scop.). The ethyl acetate extract (EaE) exhibited the highest polyphenols (57.60 ± 0.17 μg GAE/mg) and flavonoids (49.46 ± 0.66 μg QE/mg), while the methanolic extract (ME) demonstrated the strongest DPPH scavenging activity (IC₅₀ = 56.05 ± 0.03 µg/ml) and β-carotene bleaching inhibition, comparable to BHT. The aqueous and ethyl acetate extracts showed the highest total antioxidant capacity (252.60 ± 0.20 and 249.10 ± 0.81 µg AAE/mg, respectively). Antifungal assays revealed that ethyl acetate and methanolic extracts were the most effective, with inhibition percentages (IP) exceeding 65% against all tested fungi. Additionally, the methanolic extract at 30% concentration induced 100% mortality in A. fabae after 72 h and exhibited significant repellency (48.98 ± 8.76%). These findings highlight O. glabrum as a promising source of natural biopesticides for integrated pest management.
Citation
ABDALLAH KHERBACHE , MOHAMMED Allouani , NOUI Hendel , MADANI Sarri , Djamel SARRI , Dahou Moutassem, Meriem Sahraoui, , (2025-09-30), Otospermum glabrum extracts: antioxidant properties and bioactivity against fungal pathogens and Aphis fabae, Plant Protection Bulletin, Vol:65, Issue:3, pages:69 - 81, TARIM VE ORMAN BAKANLIĞI EĞİTİM VE YAYIN DAİRESİ BAŞKANLIĞI
- 2025-09-28
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2025-09-28
Traditional Applications, Phytochemical Constituents, and Pharmacological Properties of Lavandula multifida L.: A Review
The genus Lavandula represents one of the most valuable aromatic and medicinal plants, holding significant economic importance in the pharmaceutical, food, perfumery, and cosmetics industries. Among them, L. multifida is a traditionally used medicinal plant in the Mediterranean region. This work provides a comprehensive review of L. multifida, focusing on its traditional uses, phytochemistry, and pharmacological properties. Unlike conventional lavenders, its essential oil is dominated by phenolic monoterpenes, principally carvacrol, alongside significant concentrations of β-bisabolene, 1,8-cineole, and camphor. This distinct phytochemical profile is further complemented by a rich range of non-volatile constituents, including flavonoids, phenolic acids, and triterpenoids. Pharmacological investigations have validated its broad-spectrum antimicrobial activity, demonstrating efficacy against multidrug-resistant bacterial strains and fungal pathogens through mechanisms such as membrane disruption, metabolic interference, and quorum sensing inhibition. Furthermore, the species exhibits significant antioxidant and anti-inflammatory properties, mediated primarily through radical scavenging, cyclooxygenase inhibition, and cytokine modulation. Owing to its distinct chemistry, specific traditional uses for respiratory and digestive ailments, limited endemic habitat, and underexplored status, L. multifida presents a promising candidate for future research with high potential for novel drug discovery, particularly in antiparasitic and respiratory therapies. This review concludes by identifying key research priorities for L. multifida, including a detailed analysis of its non-volatile compounds, mechanistic elucidation, toxicological assessments, and standardization of extracts. Addressing these gaps is essential to validate its traditional applications and advance its development into evidence-based phytomedicines, adjuvant therapies, and natural agrochemicals. Keywords: Lavandula multifida; phytochemistry; ethnopharmacology; bioactive compounds; mediterranean; biological activities
Citation
MADANI Sarri , MOHAMMED Allouani , NOUI Hendel , Djamel SARRI , Dahou Moutassem, Antonella D’Anneo, Giuseppe Gallo, Antonio Palumbo Piccionello, , (2025-09-28), Traditional Applications, Phytochemical Constituents, and Pharmacological Properties of Lavandula multifida L.: A Review, Molecules, Vol:30, Issue:3906, pages:1-30, MDPI (Academic Editor: Hinanit Koltai)
- 2024-05-07
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2024-05-07
Etude des activités antioxydante et antimicrobienne des extraits de Linaria scariosa (Vent.) Desf
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Citation
Djamel SARRI , NOUI Hendel , MOHAMMED Allouani , MADANI Sarri , ,(2024-05-07), Etude des activités antioxydante et antimicrobienne des extraits de Linaria scariosa (Vent.) Desf,2ème colloque national de chimie (CNC2@2024).,Msila Algérie
- 2024-05-07
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2024-05-07
Evaluation of the antimicrobial activity of Otospermum glabrum extracts
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Citation
NOUI Hendel , MOHAMMED Allouani , Djamel SARRI , MADANI Sarri , ,(2024-05-07), Evaluation of the antimicrobial activity of Otospermum glabrum extracts,Le 2ème colloque national de chimie (CNC2@2024),Université de M'sila
- 2023-11-14
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2023-11-14
THE CHEMICAL COMPOSITION, BIOLOGICAL ACTIVITIES, AND TRADITIONAL USES OF LAVANDULA MULTIFIDA L. IN THE MEDITERRANEAN.
THE CHEMICAL COMPOSITION, BIOLOGICAL ACTIVITIES, AND TRADITIONAL USES OF LAVANDULA MULTIFIDA L. IN THE MEDITERRANEAN. ALLOUANI Mohammed 1, 2*, HENDEL Noui 1, 2, MOUTASSEM Dahou 3 1Microbiology and Biochemistery Departement, Faculty of Sciences, University of M’sila 2Laboratory of Biology: Applications in Health and environment 3Laboratory of Characterization and Valorization of Natural Resources, Faculty of Nature and Life Sciences, Mohamed El Bachir El Ibrahimi University, Bordj Bou Arreridj, Algeria *E-mail : mohammed.allouani@univ-msila.dz Abstract Lavandula multifida L., commonly known as Egyptian lavender or fern-leaf lavender, is a semi-evergreen perennial shrub belonging to the Lamiaceae family. It is the only Pterostoechas section species found in abundance in the Mediterranean region thriving in poorly-evolved limestone soils and hot, arid climatic conditions. This herb is traditionally used to treat a lot of conditions, such as colds, gastrointestinal issues, asthma, arthritis, rheumatoid arthritis, hypertension, and uterine irritation. Lavandula multifida essential oil is composed of various components, including oxygenated monoterpenes, sesquiterpene hydrocarbons, monoterpene hydrocarbons, and oxygenated sesquiterpenes. Carvacrol has been shown to be the primary compound in L. multifida L. essential oil, contributing significantly to its flavor and biological activities. Many investigations have revealed the plant's biological properties, which include antibacterial, antifungal, antioxidant, and anti-inflammatory actions. The L. multifida essential oil also holds substantial value in the perfume, cosmetic, flavoring, and pharmaceutical industries. Keywords: L. multifida L., traditional uses, chemical composition, essential oils, carvacrol, biological activities.
Citation
MOHAMMED Allouani , NOUI Hendel , MOUTASSEM Dahou, ,(2023-11-14), THE CHEMICAL COMPOSITION, BIOLOGICAL ACTIVITIES, AND TRADITIONAL USES OF LAVANDULA MULTIFIDA L. IN THE MEDITERRANEAN.,1ST INTERNATIONAL SEMINAR ON ECOLOGY AND BIOTECHNOLOGY? IN MEDITERRANEAN ATMOSPHERE CLIMATE (ISEBMCE'23),FERHAT ABBAS6SETIF 1 UNIVERSITY FACULTY OF NATURE AND LIFE SCIENCE - DEPARTEMENT OF PLANT BIOLOGY AND ECOLOGY
- 2023-11-14
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2023-11-14
TOTAL PHENOLIC CONTENTS, IN VITRO ANTIOXIDANT, ANTIBACTERIAL, AND ANTIFUNGAL ACTIVITIES OF THE ALGERIAN OTOSPERMUM GLABRUM (LAG.) WILLK. EXTRACTS
TOTAL PHENOLIC CONTENTS, IN VITRO ANTIOXIDANT, ANTIBACTERIAL, AND ANTIFUNGAL ACTIVITIES OF THE ALGERIAN OTOSPERMUM GLABRUM (LAG.) WILLK. EXTRACTS ALLOUANI Mohammed 1, 2*, HENDEL Noui 1, 2, MOUTASSEM Dahou 3 1Microbiology and Biochemistery Departement, Faculty of Sciences, University of M’sila 2Laboratory of Biology: Applications in Health and environment 3Laboratory of Characterization and Valorization of Natural Resources, Faculty of Nature and Life Sciences, Mohamed El Bachir El Ibrahimi University, Bordj Bou Arreridj, Algeria *E-mail : mohammed.allouani@univ-msila.dz ABSTRACT Medicinal plants include diverse plant types used in herbalism, many of which have medicinal properties. They serve as valuable resources for drug development and synthesis. Otospermum glabrum (Lag.) Willk., belonging to the Asteraceae family, is known by its synonyms, including Matricaria glabra (Lag.) Ball, Pyrethrum glabrum Lag., and Otocarpum glabrum (Lag.) Willk. This plant is also recognized as a species of chamomile and is widely used in traditional medicine. The objective of this study is to determine the total phenols and the total flavonoids contents and to evaluate in vitro the antioxidant, antibacterial, and antifungal activities of the methanolic, ethyl acetate, and aqueous extracts prepared from the aerial parts of the Algerian Otospermum glabrum (Lag.) Willk. The total phenolic and flavonoid contents were determined spectrophotometrically. The antioxidant activity of the extracts was examined by two different methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the β-Carotene bleaching test. The antibacterial activity was achieved through the well diffusion technique against seven bacterial strains, and the antifungal activity of the methanolic extract was determined using the contact assay against eight fungal strains. The results showed that the ethyl acetate extract presented the highest total phenols and total flavonoids contents (65.641±1.419 µg GAE/mg extract and 51.539± 0.468 µg QE/mg extract) compared with the metabolic (49.163±0.834 µg GAE/mg extract and 21.767± 0.623 µg QE/mg extract) and aqueous extracts (19.654±0.287 µg GAE/mg extract and 8.491± 0.078 µg QE/mg extract). The methanolic extract displayed the highest antioxidant activity in both DPPH and β-Carotene bleaching tests, with IC50 values of 53.217±0.428 and 2.725 ± 0.235, respectively. When compared to the standard antioxidant BHT, all extracts exhibit lower activity. In general, the tested bacteria can be characterized as sensitive, with inhibition diameters ranging from 9.65 to 14.85 mm, except for E. faecalis, which exhibited resistance to the tested extracts. The antifungal activity showed a significant inhibition varying from 25% to 50%, according to the tested fungal species, of which Penicillium expansum stain was the most sensitive with an inhibition of 50%. Key Words : Otospermum glabrum, phenolic contents, antioxidant activity, antibacterial activity, antifungal activity.
Citation
MOHAMMED Allouani , NOUI Hendel , MOUTASSEM Dahou, ,(2023-11-14), TOTAL PHENOLIC CONTENTS, IN VITRO ANTIOXIDANT, ANTIBACTERIAL, AND ANTIFUNGAL ACTIVITIES OF THE ALGERIAN OTOSPERMUM GLABRUM (LAG.) WILLK. EXTRACTS,1ST INTERNATIONAL SEMINAR ON ECOLOGY AND BIOTECHNOLOGY? IN MEDITERRANEAN ATMOSPHERE CLIMATE (ISEBMCE'23),FERHAT ABBAS6SETIF 1 UNIVERSITY FACULTY OF NATURE AND LIFE SCIENCE - DEPARTEMENT OF PLANT BIOLOGY AND ECOLOGY
- 2022
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2022
IMPORTANCE DU GENRE LAVANDULA EN ALGERIE
Depuis l’antiquité, les grandes civilisations ont eu recours aux plantes médicinales pour leurs propriétés diverses. L’Algérie, grâce à sa position géographique au centre de la méditerranée, est considérée comme une source très riche et diversifiée de plantes aromatiques. Parmi ces plantes aromatique la lavande. Le genre Lavandula est un membre de la famille des Lamiaceae. Les espèces de ce genre sont des arbustes aux sous-arbrisseaux avec un large éventail de parfums. De nombreuses espèces de ce genre sont rencontrées dans la flore algérienne : L. stoechas L., L. multifida L., L. dentata L et L. antineae Maire, qui poussent spontanément dans tout le pays. La Lavande occupe une place significative en médecine traditionnelle Algérienne ; elle peut être utilisée comme agent antiseptique, stimulant, et pour le traitement de rhume et d’hypotension. De nombreuses études ont montré que les huiles essentielles ou les extraits de lavande présentent une large gamme d’activités biologiques : antimicrobienne, anti-inflammatoire, antioxydante, sédative et insecticide. Les espèces de Lavandula sont des plantes aromatiques et médicinales très utiles avec une valeur économique très importante pour les industries pharmaceutique, alimentaire, aromatisante et cosmétique. Keywords : Lavandula, Algérie, utilisation traditionnelle, activités biologiques.
Citation
MOHAMMED Allouani , NOUI Hendel , ,(2022), IMPORTANCE DU GENRE LAVANDULA EN ALGERIE,INTERNATIONAL SEMINAR ON VALORIZATION OF AGRONOMIC, ECOLOGICAL AND FOOD RESOURCES(ISVAEFR 2022)18,19&20 OCTOBER 2022,University august 20,1955 Skikda
- 2022
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2022
The genus Lavandula : a promising pharmacological power
The Mediterranean region is characterized by a diverse vegetation cover, and the Lavandula genus is one of the most important medicinal and aromatic plants in this region. The genus Lavandula is composed of approximately 40 species, many hybrids, and nearly 400 registered cultivars. The best-known and most economically valuable species are L. angustifolia, L. stoechas, L. latifolia and the L. x intermedia hybrid. The Lavandula species are sub-shrubs or sometimes perennial shrubs up to one meter in height and grow in rocky and calcareous areas. Plants of the genus Lavandula are traditionally believed to be antibacterial, anti-fungal, carminative, sedative, anti-depressive, and effective for burns and insect bites. They were also prescribed for the treatment of epilepsy and migraine attacks. Phytochemical research has shown that the genus Lavandula contains a variety of secondary metabolites, mainly monoterpenes and sesquiterpenes found in essential oils. The main constituents of these essential oils are linalol, linalyle acetate, 1,8-cineole, camphor, β-ocimene and terpinen-4-ol. According to recent research, the essential oils of Lavandula species are effective in food conservation and insect control. The medical and pharmacological qualities of Lavandula plants are mainly due to its essential oils, in particular the main constituents of essential oils, although some activities have been attributed to phenolic compounds. Lavender oil is used in a variety of medicinal applications; it is considered an oral antispasmodic, soporific agent, anxiolytic agent, chemotherapeutic agent, topical antibiotic, and a treatment for alopecia. Keywords: Lavandula, medicinal uses, essential oils, biological activities.
Citation
MOHAMMED Allouani , NOUI Hendel , Dahou Moutassem, ,(2022), The genus Lavandula : a promising pharmacological power,SUBSTANCE NATURELLE AUJOURD4HUI; MEDICAMENT PROMETTEUR DEMAINPremier séminaire national "webinaire" sur les substances bioactive. 13-14 décembre 2022,Université Mohamed Boudiaf - M'sila