NABILA Adoui

SAOUDI Nourelhouda. , MAATOUG Salsabil

Contribution à l’étude phytochimique et activité biologique d’une plante médicinale Mentha suaveolens.

BEN CHAABI Zineb , BEN MAHDJOUBA Rahma, BEN AMER Malika

Valorisation phytochimique d'une plante médicinale Alchimilla vulgaris L.

Laidi Chayma , Djabari kaouther, Laadjel ferial

alorisation phytochimique de l’extrait aqueux de la plante Malva sylvestris L.

BAKRI Samah , DILMI Fatima zohra

Etude ethnobotanique de la plante médicinale Bunium Mauritanium (Talghouda) dans la région de M’sila

ZEGHLACHE, Mariya Turkiya. , ZID ELKHIR, Leyla

Etude Ethnobotanique des plantes médicinales utilisées dans le domaine cosmétique et dermatologique dans la région de M’Sila (L’Est Algérien)

This study comprehensively analyzed the phytochemical profile, antioxidant activity, and enzyme-inhibitory properties of Rumex vesicarius (R. vesicarius) extracts using advanced food analytical methods. The methanol extract was profiled using high-performance liquid chromatography with diode array detector (HPLC–DAD), identifying 16 phenolic compounds, with cynarin (26.06 mg/g dry weight extract) as the most abundant compound, revealed for the first time in this species. The hexane extract was assessed by gas chromatography-mass spectrometry (GC–MS), revealing 12 compounds, predominantly linoleic acid (46.09%). Mineral content was measured using inductively coupled plasma mass spectrometry (ICP-MS), showing high concentrations of iron (497.03 mg/L) and calcium (3.31%). The methanol extract exhibited strong antioxidant activity, with IC 50 values of 47.25 µg/mL (DPPH assay) and 26.98 µg/mL (ABTS assay) and an A0.5 value of 33.76 µg/mL in the reducing power assay. The hexane extract exhibited significant enzyme-inhibitory activity against α-amylase (IC50=33.80 µg/mL) and α-glucosidase (IC50 = 154.19 µg/mL). Additionally, ADME/T (absorption, distribution, metabolism, excretion, and toxicity) analysis of the HPLC–DAD-detected compounds indicated favorable drug-likeness and pharmacokinetic properties. These findings underline the promise of R. vesicarius as a rich source of bioactive compounds with antioxidant and antidiabetic properties, making it a promising candidate for further research in functional foods. The use of robust analytical methods, including HPLC–DAD, GC–MS, and ICP-MS, ensures reliable and reproducible results, aligning with the standards of food analytical methodologies.

Citation

Nabila ADOUI , Khellaf REBBAS , Hamdi Bendif, Imane Haouame, Dilaycan Cam, Fehmi Boufahja, Mehmet Öztürk, Stefania Garzoli, , (2025-04-10), Unveiling the Phytochemical Profiling by Chromatographic and Spectrometric Techniques and the Bioactive Potential of Rumex vesicarius L., Food Analytical Methods, Vol:1, Issue:1, pages:1-12, Food Analytical Methods

ABSTRACT This study biodiversity of the use of medicinal plants among the population of eastern Algeria for the treatment of irritable bowel syndrome (IBS), a prevalent gastrointestinal disorder. A total of 210 respondents, primarily women (63%), participated in the survey, revealing a significant inclination towards traditional remedies, particularly among individuals over 40;years old and those with no formal education (70%). The findings indicate that 70% of the participants reported using medicinal plants, with a notable preference for oral administration due to its patient-friendly nature and effectiveness in addressing internal disorders. The research highlights the predominant use of aerial parts of plants, with decoction being the most common preparation method, facilitating the extraction of active compounds. The study also emphasizes the socio-demographic profile of medicinal plant users, noting that married individuals constitute 70% of the respondents, reflecting their role as primary caregivers. The results suggest that the local population's reliance on traditional remedies stems from accumulated knowledge passed down through generations, although there is a concerning decline in this knowledge among younger individuals. The research also revealed the presence of 35 medicinal plants categorized into 21 families, with Apiaceae and Lamiaceae being the predominant ones. This research contributes to the understanding of the cultural significance and therapeutic potential of medicinal plants in Algeria, advocating for further clinical studies to validate their efficacy in treating IBS. By documenting local practices and preferences, this study aims to preserve traditional knowledge and promote the integration of herbal medicine into modern healthcare approaches, ultimately enhancing patient care and treatment options for IBS.

Citation

Nabila ADOUI , , (2024-12-01), Irritable bowel syndrome: ethnobotanical survey of medicinal plants used by the people of eastern Algeria, Brazilian Journal of Health Review, Vol:7, Issue:9, pages:01-23, Brazilian Journal of Health Review, Curitiba,

phytochimistry and biological activities of euphorbiaceae from the algerian sahara

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Nabila ADOUI , ,(2023-10-24), phytochimistry and biological activities of euphorbiaceae from the algerian sahara,First international seminar on substuniable managment of natural resources in arid and semi arid areas,Naama

Abstract: Pallenis spinosa (L.) Cass. is a widespread plant in the Mediterranean region. Traditionally, it is used as a medicinal species to treat several ailments, from inflammation to skin injuries. Although the phytochemical content of this plant has already been investigated, there is currently limited data on Algerian P. spinosa. In this work, we focused on volatile compounds and non-volatile secondary metabolites extracted using HS-SPME and methanol from the aerial parts of P. spinosa collected from Northeast Algeria. Volatile constituents were analyzed by GC-MS, while non-volatile compounds were analyzed by NMR and HPLC-MSn . In total, 48 volatile compounds were identified, including sesquiterpene hydrocarbons (65.8%), monoterpene hydrocarbons (16.9%), and oxygenated monoterpenes and sesquiterpenes (8.3% and 6.5%, respectively). β-Chamigrene (16.2%), α-selinene (12.8%), β-pinene (10.6%), and β-caryophyllene (9.2%) were assessed as the main constituents. Concerning non-volatile metabolites, 23 polyphenols were identified (7.26 mg/g DW), and phenolic acids were predominant (5.83 mg/g DW). Tricaffeoylhexaric acid (1.76 mg/g DW), tetracaffeoylhexaric acid (1.41 mg/g DW), 3,5-dicaffeoylquinic acid (1.04 mg/g DW), caffeoyl dihexoside (0.35 mg/g DW), and chlorogenic acid (0.29 mg/g DW) were the most abundant ones. Several known flavonoids, such as tricin and patuletin glycosides, kaempferol, and apigenin, were also identified, and myricetin hexoside was detected in P. spinosa for the first time. Overall, our work is the first to report an exhaustive characterization of volatile and non-volatile secondary metabolites from Algerian P. spinosa. The results represent a step forward in revealing the chemistry of this widespread plant species. Furthermore, they may contribute to rationalizing its traditional medicinal applications and preserve the biodiversity of Algerian flora. Keywords: Pallenis spinosa; secondary metabolites; terpenes; polyphenols; headspace analysis; mass spectrometry

Citation

HAMDI Bendif , Nabila ADOUI , , (2023-09-08), Characterization of Polyphenols and Volatile Compounds from Understudied Algerian Pallenis spinosa by HS-SPME-GC-MS, NMR and HPLC-MSn, Appl. Sci., Vol:13, Issue:1, pages:13, MDPI

Characterization of Polyphenols and Volatile Compounds of the Algerian White Thorn Under Study by HS-SPME-GC-MS, NMR and HPLC-MSn Methods”

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Nabila ADOUI , ,(2023-05-04), Characterization of Polyphenols and Volatile Compounds of the Algerian White Thorn Under Study by HS-SPME-GC-MS, NMR and HPLC-MSn Methods”,7th International Conference on Mass Spectrometry & Separation Techniques”,ONLINE WEBINAR

Introduction: Verbascum L. is the largest genus of the Scrophulariaceae family, which comprises more than 300 species of wild growing plants, Verbascum betonicifolium (V. betonicifolium) is a plant used in traditional medicine for several ailments. The objective of this study was to determine the first phytochemical characterization of aqueous extracts from aerial parts. Methods: The compounds present in the aerial part aqueous extract were identified by high performance liquid chromatography, coupled to a diode-array detector (HPLC-DAD) and by high-resolution mass spectrometry (HRMS), using LC–MS/MS analyses. Results: Six types of plant metabolites were found: primary metabolites like malic, gluconic and citric acids, representing 36 % of the identified compounds; secondary metabolites like flavonoid derivatives (apigenin, luteolin) and several glycosylated derivatives, representing 27 % of the identified compounds; caffeic acid derivatives (chlorogenic acid) and several flavonoid derivatives representing 9 % of the identified compounds; iridoids like methylscutelloside, scropheanoside, saccatoside and so on, representing 30 % of the identified compounds; one lignan and a lactone sesquiterpenoid were also tentatively suggested.Conclusions: These results show for the first time the richness of phytochemicals of V. betonicifolium and that the aqueous extract could be used as new natural sources of bioactive molecules.

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HAMDI Bendif , Hanane KHALFA , Nabila ADOUI , Karim MERABTI , LARBI Derbak , Imane Haouame , ,(2023), IRIDOIDS AND FLAVONOIDS PRESENT IN DECOCTIONS FROM AERIAL PARTS OF VERBASCUM BETONICIFOLIUM,Séminaire national sur Les Substances Bioactives (SBIO-2023) 14 Mars 2023,univ de ghardaia

Phytochemistry and biological activities of an Euphorbiaceae from the Algerian Sahara.

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HAMDI Bendif , Nabila ADOUI , Khellaf REBBAS , ,(2023), Phytochemistry and biological activities of an Euphorbiaceae from the Algerian Sahara.,First international seminar on sustainable management of natural ressouces in arid and semi arid areas.,Université Naama

Abstract: The purpose of this review is to provide comprehensive scientific information on Talghouda (Bunium fontanesii), the geographical distribution, botanical description, its chemistry, pharmacological properties of species are stated. As much data on B. fontanesii was gathered using several scientific search engines as: Google Scholar, Science Direct, PubMed, SpringerLink, Web of Science and Scopus. In this review, studies mentioned B. fontanesii and related synonyms and its phytopharmacological activities were cited, the data have been classified according to the ethnomedical use, the phytochemistry and the biological activities of Talghouda. Previous ethnobotanical studies have reported that Talghouda has been used in many folk medicines, especially in thyroid dysfunction, their essential oils and seeds are used in foods in the form of bread. Compositional analysis in different parts of B. fontanesii shows different classes of compounds, including terpenoids and non-terpenoids, as caryophyllene oxide and caryophyllene, (Z)-farnesene, germacrene B, spathulenol, nonacosane and palmitic acid. Phenolic compounds, fatty acids and alkaloids. Polar and non-polar extracts like essential oils have a wide variety of pharmacological activities, i.e., antioxidant, antifungal, antibacterial, anti-inflammatory, anticancer, enzyme inhibition, antiglycation, phytotoxic activity, hypoglycemic effect, estrogenic effects activities. This review confirms the important ethnobotanical and pharmacological potential of Talghouda.

Citation

Nabila ADOUI , , (2022-03-01), ETHNOMEDICINAL USES, PHYTOCHEMISTRY AND BIOLOGICAL ACTIVITIES OF TALGHOUDA (BUNIUM FONTANESII BATT. AND RELATED SYNONYMS): A REVIEW, Journal of EcoAgriTourism, Vol:18, Issue:1, pages:76-96, Journal of EcoAgriTourism

Actuellement, les plantes médicinales (PM) représentent une source considérable et permanente pour les principes actifs. Parmi les plantes médicinales industrialisées et commercialisées en Algérie, on trouve le Cuminum cyminum, Ocimum basilicum, Pimpinella anisum et Mentha pulegium. Parmi les mesures qui pourraient contribuer au redressement de ce secteur, nous citons l’instauration des surfaces cultivées des plantes médicinales. Ainsi, le semis, le bouturage et la culture in vitro qui pourraient être un moyen de production et d’approvisionnement en plantes médicinales. Ce travail expérimental sur la performance germinative des graines de C. cyminum, O. basilicum et P. anisum, dans le but de déterminer le taux de germination et la croissance dans 5semaines sans traitement dans la serre et avec traitement in vitro. Selon les espèces, les taux de germination presque sont homogènes dans les 2 compartiments de P. anisum et C. cyminum obtenu pour une durée de germination de 2 à 5 semaine tandis que l’O. basilicum a montré un retard dans la germination au cours de 3 premières semaines dans la serre, contrairement in vitro,ou il’a montré dès la 1ère semaine, et cela indique la nécessité de traiter les graines pour raccourcir le temps de germination. Ce travail expérimental aussi, a pour but la multiplication de M. pulegium par bouturage et culture in vitro, la culture in vitro donne des bons résultats concernant la multiplication de M. pulegium selon les paramètres de croissance (tige, racine, feuille et nœud) par rapport au bouturage.

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HAMDI Bendif , Nabila ADOUI , BAHLOULI Imane, BRANTI Sylia, YAHIA Ahlam, HECHAICHI Fatima Zohra, ,(2022), Effects of pre-treatment on seeds germination in vitro and in soil of some medicinal plants,1er Séminaire International sur la Biotechnologie Verte et Sécurité Alimentaire (BVSA, webinaire).,Université de KHENCHELA

valorisation des resources phylogénétique de blés dure( triticum durum desf.) cultivé en Algérie

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Nabila ADOUI , ,(2021-11-10), valorisation des resources phylogénétique de blés dure( triticum durum desf.) cultivé en Algérie,la 1 er journées scientifique nationale sur la conservation et la valorisation d la biodiversité végétale de l Algérie,SIDI BELABES

Eude quantitative des composés phénolique de Nepeta algeriensis

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Nabila ADOUI , ,(2021-04-28), Eude quantitative des composés phénolique de Nepeta algeriensis,International Seminar on Biodiversity, Valorization and Conservation of Urban and Forest Ecosystems: (In support of sustainable development),M'sila

comparaison intra et inter varietale des protéines de réserves des génotypes de quelques varietes de blé dure (triticum durum DESf)

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Nabila ADOUI , ,(2019-01-01), comparaison intra et inter varietale des protéines de réserves des génotypes de quelques varietes de blé dure (triticum durum DESf),GENOME AND WHEAT SEQUENCING,UFM Constantine

Abstract The molecular polymorphism of the stock proteins, namely, glutenin and gliadin has been studied in order to perform a comparison of the genes coding for these factors in the quality of bread as much as they are major contributors to the elasticity and formation of wheat dough. DNA markers for quality traits are currently being used by wheat breeders to directly measure traits and performing biochemical tests. The aim of this study was to use DNA markers to search for varieties with glutenin genes (HMW and LMW) and gliadin genes also. 4 durum wheat varieties (alexandrium, murcenese, italicum and valencia) were used for these genes using PCR/RFLP markers via (F-56) and (R-56) primers. Results obtained show that polymorphism rate of this two primer is 54.54%. These two primers produce the same number of amplifiers whose size is 536bp. A total of eleven fragments are obtained for each primer. Five are relatively polymorphic, which concerns the genotype: 4, 20, 76, 91 and 92 through the pairing of an expression density materialized by low intensity bands. The study also revealed that there was no product at 527bp, the size of (HMW) glutenin gene; this indicates that the 100 genotypes do not contain this gene. Thus indicating its poverty in this element; compared to the (LMW) subunit of this gene which accounts for about 60% of glutenin proteins. While there is 770bp product for the gliadin gene, which indicates also that the 100 genotypes contain this gene. Thus indicating its good quality for bread making.

Citation

Nabila ADOUI , , (2017-12-01), Analyse Moléculaire De La Diversité Génétique Des Protéines De Réserves Chez Quatre Variétés De Blé Dur (Triticum turgidum L.var durum), European Scientific Journal, Vol:13, Issue:36, pages:249-265, European Scientific Journal